| MicroRNA-195 regulates vascular smooth muscle cell phenotypes and prevents neointimal formation. | |
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MedLine Citation:
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PMID: 22802111 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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AIMS: Proliferation and migration of vascular smooth muscle cells (VSMCs) can cause atherosclerosis and neointimal formation. MicroRNAs have been shown to regulate cell proliferation and phenotype transformation. We discovered abundant expression of microRNA-195 in VSMCs and conducted a series of studies to identify its function in the cardiovascular system.Methods and ResultsMicroRNA-195 expression was initially found to be altered when VSMCs were treated with oxLDL via a single experiment of microRNA array without replication. Using cellular studies, we found that microRNA-195 reduced VSMC proliferation, migration and syntheses of interleukin(IL)-1β, IL-6 and IL-8. Using bioinformatic prediction and experimental studies, we showed that microRNA-195 could repress the expression of Cdc42, CCND1, and FGF1 genes. Using the rat model, we found that microRNA-195 gene carried by adenovirus substantially reduced the neointimal formation in the balloon-injured carotid artery. In situ hybridization confirmed the presence of microRNA-195 in the treated arteries but not in the placebo treated arteries. The experiment of immunohistochemistry for Cdc42 protein showed abundant Cdc42 in the neointima of treated arteries. CONCLUSIONS: We identified that microR-195 plays a role in the cardiovascular system by inhibiting VSMC proliferation, migration and proinflammatory biomarkers. MicroRNA-195 may have the potential to reduce the neointimal formation in patients receiving stenting or angioplasty. |
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Authors:
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Yung-Song Wang; Hay-Yan J Wang; Yi-Chu Liao; Pei-Chien Tsai; Ku-Chung Chen; Hsin-Yun Cheng; Ruey-Tay Lin; Suh-Hang Hank Juo |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-16 |
Journal Detail:
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Title: Cardiovascular research Volume: - ISSN: 1755-3245 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0077427 Medline TA: Cardiovasc Res Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Genome Medicine, Kaohsiung Medical University, No. 100, TzYou First Road, Kaohsiung, 80708, Taiwan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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