| MicroRNA 17-92 expressed by a transposone-based vector changes expression level of cell-cycle-related genes. | |
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MedLine Citation:
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PMID: 22731656 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The miR-17-92 cluster is composed of seven miRNAs (microRNAs; miR-17-5p, miR-17-3p, miR-18a, miR-19a, miR-20a, miR-19b-1 and miR-92a-1). Previous studies have indicated that this cluster is involved in cell proliferation and their overexpression has been seen in several types of cancer. We have assessed the overexpression effects of miR-17-92 on the expression of several genes associated with cell-cycle regulation. The human miR-17-92 gene was cloned into a transposone-based vector, piggyBac and transfected into HEK-293T [HEK-293 cells (human embryonic kidney cells) expressing the large T-antigen of SV40 (simian virus 40)] cell line. Gene expression analysis indicated that up-regulation of this cluster causes significant changes in the expression of several cell-cycle related genes, including CDK2 (cyclin-dependent kinase 2), cyclin-D2, c-Myc and CREB (cAMP-response-element-binding protein). Other methods of transcripts assessment confirmed miR-17-92 overexpression enhances cell proliferation. |
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Authors:
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Marzieh Attar; Ehsan Arefian; Mohammad Nabiuni; Fatemeh Jamshidi Adegani; Seyed Hamid Aghaee Bakhtiari; Zahra Karimi; Mansoureh Barzegar; Masoud Soleimani |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell biology international Volume: 36 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-10-18 Completed Date: 2013-03-21 Revised Date: 2013-03-26 |
Medline Journal Info:
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Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
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Languages: eng Pagination: 1005-12 Citation Subset: IM |
Affiliation:
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Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Polyomavirus Transforming
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genetics,
metabolism Cell Cycle* Cell Proliferation Cell Survival Cloning, Molecular Cyclic AMP Response Element-Binding Protein / genetics, metabolism Cyclin D2 / genetics, metabolism Cyclin-Dependent Kinase 2 / genetics, metabolism DNA Transposable Elements Escherichia coli / genetics, metabolism Gene Expression Profiling / methods Gene Expression Regulation, Neoplastic* Genes, Neoplasm Genetic Vectors / genetics, metabolism* HEK293 Cells Humans MicroRNAs / genetics, metabolism* Multigene Family Plasmids / genetics, metabolism Proto-Oncogene Proteins c-myc / genetics, metabolism Simian virus 40 / genetics, metabolism Transfection Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Polyomavirus Transforming; 0/CCND2 protein, human; 0/CREB1 protein, human; 0/Cyclic AMP Response Element-Binding Protein; 0/Cyclin D2; 0/DNA Transposable Elements; 0/MIRN17-92 microRNA, human; 0/MYC protein, human; 0/MicroRNAs; 0/Proto-Oncogene Proteins c-myc; EC 2.7.11.22/CDK2 protein, human; EC 2.7.11.22/Cyclin-Dependent Kinase 2 |
| Comments/Corrections | |
Erratum In:
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Cell Biol Int. 2012 Dec 1;36(12):1299 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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