| MicroRNA-155 is a negative regulator of activation-induced cytidine deaminase. | |
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MedLine Citation:
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PMID: 18450484 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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B lymphocytes perform somatic hypermutation and class-switch recombination (CSR) of the immunoglobulin locus to generate an antibody repertoire diverse in both affinity and function. These somatic diversification processes are catalyzed by activation-induced cytidine deaminase (AID), a potent DNA mutator whose expression and function are highly regulated. Here we show that AID was regulated posttranscriptionally by a lymphocyte-specific microRNA, miR-155. We found that miR-155 was upregulated in murine B lymphocytes undergoing CSR and that it targeted a conserved site in the 3'-untranslated region of the mRNA encoding AID. Disruption of this target site in vivo resulted in quantitative and temporal deregulation of AID expression, along with functional consequences for CSR and affinity maturation. Thus, miR-155, which has recently been shown to play important roles in regulating the germinal-center reaction, does so in part by directly downmodulating AID expression. |
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Authors:
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Grace Teng; Paul Hakimpour; Pablo Landgraf; Amanda Rice; Thomas Tuschl; Rafael Casellas; F Nina Papavasiliou |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't Date: 2008-05-01 |
Journal Detail:
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Title: Immunity Volume: 28 ISSN: 1097-4180 ISO Abbreviation: Immunity Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-05-16 Completed Date: 2008-07-07 Revised Date: 2011-12-13 |
Medline Journal Info:
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Nlm Unique ID: 9432918 Medline TA: Immunity Country: United States |
Other Details:
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Languages: eng Pagination: 621-9 Citation Subset: IM |
Affiliation:
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Laboratory of Lymphocyte Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3' Untranslated Regions Animals B-Lymphocytes / enzymology*, immunology, metabolism Cells, Cultured Cytidine Deaminase / genetics*, metabolism* Immunoglobulin Class Switching Lipopolysaccharides / immunology Mice Mice, Mutant Strains Mice, Transgenic MicroRNAs / genetics, immunology, metabolism* Mutation RNA, Messenger / genetics, metabolism Somatic Hypermutation, Immunoglobulin |
| Grant Support | |
ID/Acronym/Agency:
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CA098495/CA/NCI NIH HHS; GM066699/GM/NIGMS NIH HHS; R01 CA098495-05/CA/NCI NIH HHS; R01 CA098495-09/CA/NCI NIH HHS; Z01 AR041149-03/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/3' Untranslated Regions; 0/Lipopolysaccharides; 0/MicroRNAs; 0/RNA, Messenger; EC 3.5.4.-/AICDA (activation-induced cytidine deaminase); EC 3.5.4.5/Cytidine Deaminase |
| Comments/Corrections | |
Comment In:
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Immunity. 2008 May;28(5):596-8
[PMID:
18482561
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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