Document Detail

MicroRNA-155 regulates angiotensin II type 1 receptor expression and phenotypic differentiation in vascular adventitial fibroblasts.
MedLine Citation:
PMID:  20735984     Owner:  NLM     Status:  MEDLINE    
MicroRNAs (miRNAs), which are genomically encoded small RNAs, negatively regulate target gene expression at the post-transcriptional level. Our recent study indicated that microRNA-155 (miR-155) might be negatively correlated with blood pressure, and it has been suggested that miR-155-mediated target genes could be involved in the cardiovascular diseases. Bioinformatic analyses predict that angiotensin II type 1 receptor (AT(1)R) is a miR-155 target gene. The present study investigated the potential role of miR-155 in regulating AT(1)R expression and phenotypic differentiation in rat aortic adventitial fibroblasts (AFs). Luciferase assay demonstrated that miR-155 suppressed AT(1)R 3'-UTR reporter construct activity. miR-155 overexpression in AFs did not reduce target mRNA levels, but significantly reduced target protein expression. In addition, AFs transfected with pSUPER/miR-155 exhibited reduced Ang II-induced ERK1/2 activation. miR-155 overexpression in cells attenuated Ang II-induced α-smooth muscle actin (α-SMA, produces myofibroblast) expression, but did not transform growth factor beta-1 (TGF-β1). This study demonstrated that miR-155 could have an important role in regulating adventitial fibroblast differentiation and contribute to suppression of AT(1)R expression.
Liang Zheng; Chan-Chan Xu; Wen-Dong Chen; Wei-Li Shen; Cheng-Chao Ruan; Li-Min Zhu; Ding-Liang Zhu; Ping-Jin Gao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-22
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  400     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-04     Completed Date:  2010-11-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  483-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
State Key Laboratory of Medical Genomics, Shanghai Key Laboratory of Vascular Biology and Department of Hypertension, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, China.
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MeSH Terms
Angiotensin II / metabolism,  pharmacology
Aorta, Thoracic / cytology*,  metabolism
Cell Differentiation / genetics*
Cells, Cultured
Fibroblasts / cytology*,  metabolism
Gene Expression Regulation*
Hypertension / genetics*
Luciferases / genetics
MicroRNAs / genetics,  metabolism*
Mitogen-Activated Protein Kinase 1 / metabolism
Mitogen-Activated Protein Kinase 3 / metabolism
Rats, Sprague-Dawley
Receptor, Angiotensin, Type 1 / genetics*
Reg. No./Substance:
0/MicroRNAs; 0/Receptor, Angiotensin, Type 1; 11128-99-7/Angiotensin II; EC 1.13.12.-/Luciferases; EC Protein Kinase 1; EC Protein Kinase 3

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