| MicroRNA-128 downregulates Bax and induces apoptosis in human embryonic kidney cells. | |
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MedLine Citation:
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PMID: 20924637 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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MicroRNAs (miRNAs) are short ~21-nt non-coding RNA molecules that have been shown to regulate a number of biological processes. Previous reports have shown that overexpression of miR-128 in glioma cells inhibited cell proliferation. Literature also suggests that miR-128 negatively regulates prostate cancer cell invasion. Here, we show that overexpression of hsa-miR-128, a brain-enriched microRNA, induces apoptosis in HEK293T cells as elucidated by apoptosis assay, cell cycle changes, loss of mitochondrial membrane potential and multicaspase assay. By in silico analysis, we identified a putative target site within the 3' untranslated region (UTR) of Bax, a proapoptotic member of the apoptosis pathway. We found that ectopic expression of hsa-miR-128 suppressed a luciferase reporter containing the Bax-3' UTR and reduced the levels of Bax in HEK293T cells. Taken together, our study demonstrates that overexpression of hsa-miR-128 not only induces apoptosis in HEK293T cells but also is an endogenous regulator of Bax protein. |
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Authors:
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Yogita K Adlakha; Neeru Saini |
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Publication Detail:
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Type: Journal Article Date: 2010-09-19 |
Journal Detail:
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Title: Cellular and molecular life sciences : CMLS Volume: 68 ISSN: 1420-9071 ISO Abbreviation: Cell. Mol. Life Sci. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9705402 Medline TA: Cell Mol Life Sci Country: Switzerland |
Other Details:
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Languages: eng Pagination: 1415-28 Citation Subset: IM |
Affiliation:
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Functional Genomics Unit, Institute of Genomics and Integrative Biology, Council of Scientific and Industrial Research (CSIR), Mall Road, Delhi, 110007, India. |
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