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MicroRNA-126 expression is decreased in cultured primary chicken hepatocytes and targets the sprouty-related EVH1 domain containing 1 mRNA.
MedLine Citation:
PMID:  23776277     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The microRNA-126 (miR-126) is a miRNA expressed in highly vascularized tissues, and it is believed to play a role in angiogenesis by repressing sprouty-related EVH1 domain containing 1 (Spred1). In the current study, we determined the expression pattern of chicken miR-126 (gga-miR-126) and predicted and validated its target genes. The quantitative reverse-transcription (qRT) PCR analysis showed that miR-126 was expressed in various chicken tissues with the highest level in lung. In liver, the expression level of miR-126 increased from 0 to 7 wk of age. The expression of miR-126 in primary chicken hepatocytes decreased with culturing. A miR-126 binding site was predicted in the 3' UTR (untranslated region) of chicken Spred1. Dual-luciferase reporter assays indicated that miR-126 could bind to the predicted site to repress the expression of Spred1. These data validate Spred1 as a target gene of chicken miR-126. These results will help further understand the function and regulation of miR-126 and Spred1 in chickens.
Authors:
Xing-Guo Wang; Fang Shao; Hui-Juan Wang; Lei Yang; Jian-Feng Yu; Dao-Qing Gong; Zhi-Liang Gu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Poultry science     Volume:  92     ISSN:  0032-5791     ISO Abbreviation:  Poult. Sci.     Publication Date:  2013 Jul 
Date Detail:
Created Date:  2013-06-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401150     Medline TA:  Poult Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1888-96     Citation Subset:  IM    
Affiliation:
College of Animal Science and Technology, Yangzhou University, Yangzhou, 225509, P.R. China; and.
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