Document Detail

MicroRNA-221 controls expression of intercellular adhesion molecule-1 in epithelial cells in response to Cryptosporidium parvum infection.
MedLine Citation:
PMID:  21236259     Owner:  NLM     Status:  MEDLINE    
Cryptosporidium parvum is a protozoan parasite that infects gastrointestinal epithelial cells and causes diarrhoeal disease in humans and animals globally. Pathological changes following C. parvum infection include crypt hyperplasia and a modest inflammatory reaction with increased infiltration of lymphocytes into intestinal mucosa. Expression of adhesion molecules, such as intercellular adhesion molecule-1 (ICAM-1), on infected epithelial cell surfaces may facilitate adhesion and recognition of lymphocytes at infection sites. MicroRNAs (miRNAs) are small RNA molecules of 23 nucleotides that negatively regulate protein-coding gene expression via translational suppression or mRNA degradation. We recently reported that microRNA-221 (miR-221) regulates ICAM-1 translation through targeting the ICAM-1 3'-untranslated region (UTR). In this study, we tested the role of miR-221 in regulating ICAM-1 expression in epithelial cells in response to C. parvum infection using an in vitro model of human biliary cryptosporidiosis. Up-regulation of ICAM-1 at both message and protein levels was detected in epithelial cells following C. parvum infection. Inhibition of ICAM-1 transcription with actinomycin D could only partially block C. parvum-induced ICAM-1 expression at the protein level. Cryptosporidium parvum infection decreased miR-221 expression in infected epithelial cells. When cells were transfected with a luciferase reporter construct covering the miR-221 binding site in the ICAM-1 3'-UTR and then exposed to C. parvum, an enhanced luciferase activity was detected. Transfection of miR-221 precursor abolished C. parvum-stimulated ICAM-1 protein expression. In addition, expression of ICAM-1 on infected epithelial cells facilitated epithelial adherence of co-cultured Jurkat cells. These results indicate that miR-221-mediated translational suppression controls ICAM-1 expression in epithelial cells in response to C. parvum infection.
Ai-Yu Gong; Guoku Hu; Rui Zhou; Jun Liu; Yaoyu Feng; Garrett A Soukup; Xian-Ming Chen
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2011-01-12
Journal Detail:
Title:  International journal for parasitology     Volume:  41     ISSN:  1879-0135     ISO Abbreviation:  Int. J. Parasitol.     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-02-28     Completed Date:  2011-06-17     Revised Date:  2014-09-11    
Medline Journal Info:
Nlm Unique ID:  0314024     Medline TA:  Int J Parasitol     Country:  England    
Other Details:
Languages:  eng     Pagination:  397-403     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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MeSH Terms
Biliary Tract / cytology,  parasitology
Cryptosporidiosis / parasitology
Cryptosporidium parvum / pathogenicity*
Epithelial Cells / metabolism*
Gene Expression Regulation*
Intercellular Adhesion Molecule-1 / genetics,  metabolism*
Jurkat Cells / parasitology
MicroRNAs / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/MicroRNAs; 126547-89-5/Intercellular Adhesion Molecule-1

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