| MicroRNA-101 targets MAPK phosphatase-1 to regulate the activation of MAPKs in macrophages. | |
| | |
MedLine Citation:
|
PMID: 21068409 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
MAPK phosphatase-1 (MKP-1) is an archetypical member of the dual-specificity phosphatase family that deactivates MAPKs. Induction of MKP-1 has been implicated in attenuating the LPS- or peptidoglycan-induced biosynthesis of proinflammatory cytokines, but the role of noncoding RNA in the expression of the MKP-1 is still poorly understood. In this study, we show that MKP-1 is a direct target of microRNA-101 (miR-101). Transfection of miR-101 attenuates induction of MKP-1 by LPS as well as prolonged activation of p38 and JNK/stress-activated protein kinase, whereas inhibition of miR-101 enhances the expression of MKP-1 and shortens p38 and JNK activation. We also found that expression of miR-101 is induced by multiple TLR ligands, including LPS, peptidoglycan, or polyinosinic-polycytidylic acid, and that inhibition of PI3K/Akt by LY294002 or Akt RNA interference blocks the induction of miR-101 by LPS in RAW264.7 macrophage cells. Moreover, treatment of cells with dexamethasone, a widely used anti-inflammatory agent, markedly inhibits miR-101 expression and enhances the expression of MKP-1 in LPS-stimulated macrophages. Together, these results indicate that miR-101 regulates the innate immune responses of macrophages to LPS through targeting MKP-1. |
| | |
Authors:
|
Qing-Yuan Zhu; Qin Liu; Jian-Xia Chen; Ke Lan; Bao-Xue Ge |
Related Documents
:
|
3262089 - Recombinant interleukin-1 alpha and recombinant tumor necrosis factor alpha synergize i... 12639839 - Effect of cd14 blockade on endotoxin-induced acute lung injury in mice. 11805229 - A novel class of endotoxin receptor agonists with simplified structure, toll-like recep... 10090849 - Diet-induced protection against lipopolysaccharide includes increased hepatic no produc... 19422799 - Atf-2 regulates lipopolysaccharide-induced transcription in macrophage cells. 7057139 - Murine serum glycoprotein gp70 behaves as an acute phase reactant. 12694389 - Oligodendrocyte injury in multiple sclerosis: a role for p53. 21282039 - Il-2: a two-faced master regulator of autoimmunity. 8558449 - The chinese herbal remedy, t2, inhibits mitogen-induced cytokine gene transcription by ... |
Publication Detail:
|
Type: Journal Article Date: 2010-11-10 |
Journal Detail:
|
Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 185 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-12-03 Completed Date: 2011-01-04 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
|
Languages: eng Pagination: 7435-42 Citation Subset: AIM; IM |
Affiliation:
|
Institute Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Anti-Inflammatory Agents / pharmacology Cell Line Dexamethasone / pharmacology Dual Specificity Phosphatase 1 / biosynthesis, immunology* Enzyme Activation / drug effects, immunology Gene Expression Regulation, Enzymologic / drug effects Immunity, Innate / drug effects, physiology* Lipopolysaccharides / pharmacology MAP Kinase Kinase 4 / immunology, metabolism Macrophages / immunology*, metabolism Mice MicroRNAs / biosynthesis, immunology* Toll-Like Receptors / agonists, immunology, metabolism p38 Mitogen-Activated Protein Kinases / immunology, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Inflammatory Agents; 0/Lipopolysaccharides; 0/MicroRNAs; 0/Toll-Like Receptors; 50-02-2/Dexamethasone; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases; EC 2.7.12.2/MAP Kinase Kinase 4; EC 3.1.3.48/Dual Specificity Phosphatase 1; EC 3.1.3.48/Dusp1 protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: M2b monocytes predominated in peripheral blood of severely burned patients.
Next Document: Oligodendrocyte-specific FADD deletion protects mice from autoimmune-mediated demyelination.