| Micro sequential injection: fermentation monitoring of ammonia, glycerol, glucose, and free iron using the novel lab-on-valve system. | |
| | |
MedLine Citation:
|
PMID: 11284328 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
|
Using an integrated lab-on-valve manifold in a microfluidic sequential injection format (microSI), automated sample processing has been developed for off-line and on-line monitoring of small-scale fermentations. Spectrophotometric assays of ammonia, glucose, glycerol, and free iron were downscaled to use micro-quantities of commercial reagents. By monitoring the reaction rate, the response curves in a stopped-flow mode generate linear calibration curves for ammonia [r2 = 1.000 (0.9% SE)], glycerol [r2 = 0.999 (1.1% SE)], glucose [r2 = 0.999 (1.1% SE)], and free iron [r2 = 0.999 (1.5% SE)]. Since sample dilution and reagent quantities are easily adjusted within the programmable SI format, the lab-on-valve system can accommodate samples over a wide concentration range (ammonia: 3-1200 ppm; glycerol: 20-120 ppm; glucose: 35-1000 ppm; and free iron: 80-400 ppm). This work demonstrates the key advantages of miniaturization through the reduction of sample and reagent use, minimizing waste and providing a compact yet reliable instrument. The lab-on-valve manifold uses a universal hardware configuration for all analyses, only requiring changes in software protocol and choice of reagents. All of these features are of particular importance to small-scale experimental fermentation where multiple analyte analyses are needed in real-time using small sample volumes. It is hoped that this first real-life application of the lab-on-valve manifold will serve not only as a model system to downscale assays in a practical fashion, but will also inspire and promote the use of the integrated microSI manifold approach for a wider range of biotechnological applications. |
| | |
Authors:
|
C H Wu; L Scampavia; J Ruzicka; B Zamost |
Related Documents
:
|
892708 - Free-flow electrophoresis. iii. an analytical version for a rapid, quantitative determi... 11544038 - Stand-off tissue-based biosensors for the detection of chemical warfare agents using ph... 18969458 - Separation and determination of aloperine, sophoridine, matrine and oxymatrine by combi... 5415218 - Portable sampler for microorganisms in incinerator stack emissions. 20144278 - Use of serial patient hemoglobin a1c differences to determine long-term imprecision of ... 10850618 - Laser-induced fluorescence detector for liquid chromatography: applications to protein ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Analyst Volume: 126 ISSN: 0003-2654 ISO Abbreviation: Analyst Publication Date: 2001 Mar |
Date Detail:
|
Created Date: 2001-04-03 Completed Date: 2001-05-17 Revised Date: 2003-10-31 |
Medline Journal Info:
|
Nlm Unique ID: 0372652 Medline TA: Analyst Country: England |
Other Details:
|
Languages: eng Pagination: 291-7 Citation Subset: - |
Affiliation:
|
Department of Chemistry, Box 351700, University of Washington, Seattle, WA 98195-1700, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Liquid chromatography with post-column electrochemical treatment and mass spectrometric detection of...
Next Document: Study of the separation and determination of monosaccharides in soluble coffee by capillary zone ele...