Document Detail

Micro-scale method for theophylline in body fluids by reversed-phase, high-pressure liquid chromatography.
MedLine Citation:
PMID:  837550     Owner:  NLM     Status:  MEDLINE    
We describe a micro-scale method for determining serum theophylline. The chromatography system includes a muBondapack C18 column and acetonitrile, 70 ml/liter of sodium acetate buffer (10 mmol/liter, ph 4.0) as the mobile phase. Test serum or plasma, 30 mul, is mixed with an equal quantity of a solution containing the internal standard, beta-hydroxyethyltheophylline in acetonitrile/sodium acetate buffer (20 mmol/liter, pH 4.0), 7/43 by vol. After the precipitate is removed by centrifugation, the mixture is chromatographed and the amount of theophylline calculated from the ratio between peak heights for theophylline and the internal standard. Advantages include easy sample preparation, involving only addition of internal standard and centrifugation before injection, long column life, and the suitability of the internal standard, which is adjusted to a peak height equivalent to 20 mg of theophylline per liter for easy computation of results.
J J Orcutt; P P Kozak; S A Gillman; L H Cummins
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical chemistry     Volume:  23     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1977-04-28     Completed Date:  1977-04-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  599-601     Citation Subset:  IM    
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MeSH Terms
Chromatography, High Pressure Liquid / methods
Saliva / analysis
Theophylline / analysis*,  blood
Reg. No./Substance:

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