Document Detail

Micro-miniature autonomous optical sensor array for monitoring ions and metabolites 2: color responses to pH, K+ and glucose.
MedLine Citation:
PMID:  16837742     Owner:  NLM     Status:  MEDLINE    
In Part 1 of this series (Anal. Sci., 2006, 22, 383), design, fabrication, and optical data acquisition of an array of tiny color changing capsules embedded in a cellulose acetate bar, called the "sliver sensor", have been described. Capsule colors are read by a CCD camera and translated into blue, red and green Kubelka-Munk variables for quantitative analysis. The respective concentrations are determined using prior calibration. The approach may be adapted to different non-biological analytical problems, as well as in vitro and in vivo applications. To demonstrate this adaptability to potential in vivo use as an example, sensitivity for each target ion was tuned to cover the respective interstitial levels by varying the relative amount of ionophore used in the corresponding microscopic beads. After optimizing the ratio of glucose oxidase (GOX)-containing beads relative to the coupled pH sensing beads and their composition, reversible color response to glucose was obtained in the entire clinically relevant glucose concentration range (10 to 600 mg/dL, 0.55 to 33 mM). Decoupling of pH and glucose sensing from possible variations in interstitial sodium level and buffer capacity is currently being optimized for future in vivo use. In vitro and non-biological applications are also being explored.
Koji Tohda; Miklós Gratzl
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  22     ISSN:  0910-6340     ISO Abbreviation:  Anal Sci     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-07-13     Completed Date:  2007-02-20     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  937-41     Citation Subset:  IM    
Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH 44106, USA.
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MeSH Terms
Biosensing Techniques / methods*
Cellulose / analogs & derivatives*,  chemistry,  metabolism
Glucose / chemistry*
Glucose Oxidase / chemistry
Hydrogen-Ion Concentration
Ions / chemistry
Optics and Photonics
Potassium / chemistry*
Sensitivity and Specificity
Reg. No./Substance:
0/Ions; 50-99-7/Glucose; 7440-09-7/Potassium; 9004-34-6/Cellulose; 9004-35-7/acetylcellulose; EC Oxidase

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