| Micro magnetic tweezers for nanomanipulation inside live cells. | |
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MedLine Citation:
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PMID: 15556976 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study reports the design, realization, and characterization of a multi-pole magnetic tweezers that enables us to maneuver small magnetic probes inside living cells. So far, magnetic tweezers can be divided into two categories: I), tweezers that allow the exertion of high forces but consist of only one or two poles and therefore are capable of only exerting forces in one direction; and II), tweezers that consist of multiple poles and allow exertion of forces in multiple directions but at very low forces. The magnetic tweezers described here combines both aspects in a single apparatus: high forces in a controllable direction. To this end, micron scale magnetic structures are fabricated using cleanroom technologies. With these tweezers, magnetic flux gradients of nablaB = 8 x 10(3) T m(-1) can be achieved over the dimensions of a single cell. This allows exertion of forces up to 12 pN on paramagnetic probes with a diameter of 350 nm, enabling us to maneuver them through the cytoplasm of a living cell. It is expected that with the current tweezers, picoNewton forces can be exerted on beads as small as 100 nm. |
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Authors:
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Anthony H B de Vries; Bea E Krenn; Roel van Driel; Johannes S Kanger |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2004-11-19 |
Journal Detail:
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Title: Biophysical journal Volume: 88 ISSN: 0006-3495 ISO Abbreviation: Biophys. J. Publication Date: 2005 Mar |
Date Detail:
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Created Date: 2005-03-01 Completed Date: 2005-07-06 Revised Date: 2010-09-21 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: 2137-44 Citation Subset: IM |
Affiliation:
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Biophysical Engineering, Faculty of Science and Technology, Institute for Biomedical Technology, University of Twente, 7500 AE Enschede, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Culture Techniques
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instrumentation*,
methods Cells, Cultured Equipment Design Equipment Failure Analysis Granulocytes / physiology* Humans Magnetics* Micromanipulation / instrumentation*, methods Microspheres Miniaturization Molecular Probe Techniques / instrumentation* Nanotechnology / instrumentation*, methods Physical Stimulation / instrumentation*, methods Stress, Mechanical |
| Comments/Corrections | |
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