| Micro-injection of Morpholino Oligonucleotides for Depleting Securin in Mouse Oocytes. | |
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MedLine Citation:
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PMID: 23138950 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Gene silencing techniques have brought new insights into mammalian oocyte and embryo development. More specifically, the use of Morpholino oligonucleotides which sterically inhibit translation from target mRNAs thereby compromising gene function, allowed the identification of important oocyte regulators and especially factors involved in meiotic cell cycle control. Here we describe the method of application of Morpholino oligonucleotides in mouse oocyte research. |
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Authors:
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Petros Marangos |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 957 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2013 |
Date Detail:
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Created Date: 2012-11-09 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 153-62 Citation Subset: IM |
Affiliation:
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Department of Biological Applications and Technology, University of Ioannina, Ioannina, Greece, pmaragos@cc.uoi.gr. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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