Document Detail


Micro-injection of Morpholino Oligonucleotides for Depleting Securin in Mouse Oocytes.
MedLine Citation:
PMID:  23138950     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Gene silencing techniques have brought new insights into mammalian oocyte and embryo development. More specifically, the use of Morpholino oligonucleotides which sterically inhibit translation from target mRNAs thereby compromising gene function, allowed the identification of important oocyte regulators and especially factors involved in meiotic cell cycle control. Here we describe the method of application of Morpholino oligonucleotides in mouse oocyte research.
Authors:
Petros Marangos
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  957     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  153-62     Citation Subset:  IM    
Affiliation:
Department of Biological Applications and Technology, University of Ioannina, Ioannina, Greece, pmaragos@cc.uoi.gr.
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