Document Detail

Micro-flow visualization of red blood cell-enhanced platelet concentration at sudden expansion.
MedLine Citation:
PMID:  18418710     Owner:  NLM     Status:  MEDLINE    
Microscopic steps and crevices are inevitable features within prosthetic blood-contacting devices. This study aimed to elucidate the thrombogenicity of the associated microscopic flow features by studying the transport of fluorescent platelet-sized particles in a suspension of red blood cells (RBCs) flowing through a 100 microm:200 microm sudden expansion. Micro-flow visualization revealed a strong influence of hematocrit upon the path of RBCs and spatial concentration of particles. At all flow rates studied (Re = 8.3-41.7) and hematocrit 20% and lower, RBC streamlines were found to detach from the microchannel wall creating an RBC-depleted zone inside the step that was much larger than the cells themselves. However, the observed distribution of particles was relatively homogeneous. By contrast, the RBC streamlines of samples with hematocrit equal to or greater than 30% more closely followed the contour of the microchannel, yet exhibited enhanced concentration of particles within the corner. The corresponding size of the cell depletion layer was comparable with the size of the cells. This study implies that local platelet concentration in blood within the physiological range of hematocrit can be elevated within the flow separation region of a sudden expansion and implicates the role of RBCs in causing this effect.
Rui Zhao; Joie N Marhefka; Fangjun Shu; Samuel J Hund; Marina V Kameneva; James F Antaki
Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-04-17
Journal Detail:
Title:  Annals of biomedical engineering     Volume:  36     ISSN:  1573-9686     ISO Abbreviation:  Ann Biomed Eng     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-05-29     Completed Date:  2008-06-27     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  0361512     Medline TA:  Ann Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1130-41     Citation Subset:  IM    
Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.
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MeSH Terms
Blood Cells / cytology,  physiology*
Blood Platelets / cytology,  physiology*
Cell Culture Techniques / instrumentation,  methods
Coculture Techniques / instrumentation,  methods
Equipment Design
Equipment Failure Analysis
Flow Cytometry / instrumentation*,  methods
Image Interpretation, Computer-Assisted / instrumentation,  methods*
Microfluidic Analytical Techniques / instrumentation*,  methods
Microscopy, Fluorescence / instrumentation*,  methods
Platelet Activation / physiology*
Grant Support

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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