| Micro-flow visualization of red blood cell-enhanced platelet concentration at sudden expansion. | |
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MedLine Citation:
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PMID: 18418710 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microscopic steps and crevices are inevitable features within prosthetic blood-contacting devices. This study aimed to elucidate the thrombogenicity of the associated microscopic flow features by studying the transport of fluorescent platelet-sized particles in a suspension of red blood cells (RBCs) flowing through a 100 microm:200 microm sudden expansion. Micro-flow visualization revealed a strong influence of hematocrit upon the path of RBCs and spatial concentration of particles. At all flow rates studied (Re = 8.3-41.7) and hematocrit 20% and lower, RBC streamlines were found to detach from the microchannel wall creating an RBC-depleted zone inside the step that was much larger than the cells themselves. However, the observed distribution of particles was relatively homogeneous. By contrast, the RBC streamlines of samples with hematocrit equal to or greater than 30% more closely followed the contour of the microchannel, yet exhibited enhanced concentration of particles within the corner. The corresponding size of the cell depletion layer was comparable with the size of the cells. This study implies that local platelet concentration in blood within the physiological range of hematocrit can be elevated within the flow separation region of a sudden expansion and implicates the role of RBCs in causing this effect. |
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Authors:
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Rui Zhao; Joie N Marhefka; Fangjun Shu; Samuel J Hund; Marina V Kameneva; James F Antaki |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-04-17 |
Journal Detail:
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Title: Annals of biomedical engineering Volume: 36 ISSN: 1521-6047 ISO Abbreviation: Ann Biomed Eng Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-05-29 Completed Date: 2008-06-27 Revised Date: 2012-12-11 |
Medline Journal Info:
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Nlm Unique ID: 0361512 Medline TA: Ann Biomed Eng Country: United States |
Other Details:
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Languages: eng Pagination: 1130-41 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blood Cells
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cytology,
physiology* Blood Platelets / cytology, physiology* Cell Culture Techniques / instrumentation, methods Coculture Techniques / instrumentation, methods Equipment Design Equipment Failure Analysis Flow Cytometry / instrumentation*, methods Humans Image Interpretation, Computer-Assisted / instrumentation, methods* Microfluidic Analytical Techniques / instrumentation*, methods Microscopy, Fluorescence / instrumentation*, methods Platelet Activation / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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R01 HL089456/HL/NHLBI NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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