| Micro-determination of nucleic acids with a simple probe manganese chloride based on the fine enhanced resonance light scattering. | |
| | |
MedLine Citation:
|
PMID: 17327143 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Manganese chloride can form large particles with nucleic acids by electrostatic forces, which results in strong enhancement of resonance light scattering (RLS) signals. Based on this phenomenon, a novel and very simple assay of DNA was established. The work conditions have been investigated including the concentration of probe, the acidity of solution, the effect of ionic strength and the selectivity. In acidic solution, the enhanced RLS intensity at 389.5 nm was proportional to the concentration of nucleic acids in the range 0.05-10.0 microg ml(-1) for both ctDNA and fsDNA and 1.0-10.0 microg ml(-1) for yRNA. The limits of detection (LOD, 3sigma) were 0.17, 0.13 and 0.53 ng ml(-1) for ctDNA, fsDNA and yRNA, respectively. Synthetic samples were determined satisfactorily. |
| | |
Authors:
|
Zhanguang Chen; Xuhong Liao; Li Zhu; Jinbin Liu; Yali Han |
Related Documents
:
|
17447763 - Ab initio study of the excited-state deactivation pathways of protonated tryptophan and... 7008833 - Esterification of terminal phosphate groups in nucleic acids with sorbitol and its appl... 713603 - Metabolism of uric acid in normal and toxemic pregnancy. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-11-24 |
Journal Detail:
|
Title: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Volume: 68 ISSN: 1386-1425 ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Publication Date: 2007 Oct |
Date Detail:
|
Created Date: 2007-09-03 Completed Date: 2007-12-19 Revised Date: 2008-11-21 |
Medline Journal Info:
|
Nlm Unique ID: 9602533 Medline TA: Spectrochim Acta A Mol Biomol Spectrosc Country: England |
Other Details:
|
Languages: eng Pagination: 263-8 Citation Subset: IM |
Affiliation:
|
Department of Chemistry, Shantou University, Shantou 515063, China. kqlu@stu.edu.cn |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cattle Chlorides / chemistry DNA / analysis Fishes Hot Temperature Hydrogen-Ion Concentration Light Male Manganese Compounds / chemistry Microchemistry / methods* Nucleic Acid Denaturation Nucleic Acids / analysis* Osmolar Concentration RNA, Fungal / analysis Scattering, Radiation Solutions Static Electricity |
| Chemical | |
Reg. No./Substance:
|
0/Chlorides; 0/Manganese Compounds; 0/Nucleic Acids; 0/RNA, Fungal; 0/Solutions; 7773-01-5/manganese chloride; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Spectral, IR and magnetic studies of Mn(II), Co(II), Ni(II) and Cu(II) complexes with pyrrole-2-carb...
Next Document: Fluorimetric and mass spectrometric study of the interaction of beta-cyclodextrin and osthole.