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Micro determination of ascorbic acid using methyl viologen.
MedLine Citation:
PMID:  19071717     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A new simple and sensitive analytical spectrophotometric method is developed for the determination of ascorbic acid reduces methyl viologen to form a stable blue coloured free radical ion. This method has a sensitivity and lower limit detection of 0.1mugml(-1) of ascorbic acid (0.1ppm) which is comparable to the flow injection analysis reported earlier. Beer's law is obeyed over the concentration range of 1.0-10mugml(-1) of ascorbic acid per 10ml of the final solution (0.1-1.0mugml(-1)) at 600nm. The molar absorptivity and Sandell's sensitivity were found to be 1.5x10(5)+/-100lmol(-1)cm(-1) and 0.001mugcm(-2), respectively. The method has been applied to the determination of ascorbic acid in food, pharmaceuticals and biological samples.
Authors:
E K Janghel; V K Gupta; M K Rai; J K Rai
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Publication Detail:
Type:  Journal Article     Date:  2007-01-08
Journal Detail:
Title:  Talanta     Volume:  72     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2007 May 
Date Detail:
Created Date:  2008-12-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  1013-6     Citation Subset:  -    
Affiliation:
Chhattisgarh Institute of Technology, Near Parri Nala, G.E. Road, Rajnangaon, Chhattisgarh, India.
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