Document Detail


A micro-delivery approach for studying microvascular responses to localized oxygen delivery.
MedLine Citation:
PMID:  21914035     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: In vivo video microscopy has been used to study blood flow regulation as a function of varying oxygen concentration in microcirculatory networks. However, previous studies have measured the collective response of stimulating large areas of the microvascular network at the tissue surface.
OBJECTIVE: We aimed to limit the area being stimulated by controlling oxygen availability to highly localized regions of the microvascular bed within intact muscle.
DESIGN AND METHOD: Gas of varying O(2) levels was delivered to specific locations on the surface of the Extensor Digitorum Longus muscle of rat through a set of micro-outlets (100 μm diameter) patterned in ultrathin glass using state-of-the-art microfabrication techniques. O(2) levels were oscillated and digitized video sequences were processed for changes in capillary hemodynamics and erythrocyte O(2) saturation.
RESULTS AND CONCLUSIONS: Oxygen saturations in capillaries positioned directly above the micro-outlets were closely associated with the controlled local O(2) oscillations. Radial diffusion from the micro-outlet is limited to ~75 μm from the center as predicted by computational modeling and as measured in vivo. These results delineate a key step in the design of a novel micro-delivery device for controlled oxygen delivery to the microvasculature to understand the fundamental mechanisms of microvascular regulation of O(2) supply.
Authors:
Nour W Ghonaim; Leo W M Lau; Daniel Goldman; Christopher G Ellis; Jun Yang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microcirculation (New York, N.Y. : 1994)     Volume:  18     ISSN:  1549-8719     ISO Abbreviation:  Microcirculation     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-21     Completed Date:  2012-03-15     Revised Date:  2014-09-22    
Medline Journal Info:
Nlm Unique ID:  9434935     Medline TA:  Microcirculation     Country:  United States    
Other Details:
Languages:  eng     Pagination:  646-54     Citation Subset:  IM    
Copyright Information:
© 2011 John Wiley & Sons Ltd.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport / physiology
Blood Flow Velocity / physiology
Capillaries / physiology*
Male
Microcirculation / physiology*
Microscopy, Video
Oxygen / metabolism*
Oxygen Consumption / physiology*
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
R33 HL-089125/HL/NHLBI NIH HHS; R33 HL089125/HL/NHLBI NIH HHS; R33 HL089125-03/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
S88TT14065/Oxygen
Comments/Corrections

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