| Micro-computed tomography (micro-CT) as a potential tool to assess the effect of dynamic coating routes on the formation of biomimetic apatite layers on 3D-plotted biodegradable polymeric scaffolds. | |
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MedLine Citation:
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PMID: 17323152 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This work studies the influence of dynamic biomimetic coating procedures on the growth of bone-like apatite layers at the surface of starch/polycaprolactone (SPCL) scaffolds produced by a 3D-plotting technology. These systems are newly proposed for bone Tissue Engineering applications. After generating stable apatite layers through a sodium silicate-based biomimetic methodology the scaffolds were immersed in Simulated Body Fluid solutions (SBF) under static, agitation and circulating flow perfusion conditions, for different time periods. Besides the typical characterization techniques, Micro-Computed Tomography analysis (micro-CT) was used to assess scaffold porosity and as a new tool for mapping apatite content. 2D histomorphometric analysis was performed and 3D virtual models were created using specific softwares for CT reconstruction. By the proposed biomimetic routes apatite layers were produced covering the interior of the scaffolds, without compromising their overall morphology and interconnectivity. Dynamic conditions allowed for the production of thicker apatite layers as consequence of higher mineralizing rates, when comparing with static conditions. micro-CT analysis clearly demonstrated that flow perfusion was the most effective condition in order to obtain well-defined apatite layers in the inner parts of the scaffolds. Together with SEM, this technique was a useful complementary tool for assessing the apatite content in a non-destructive way. |
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Authors:
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A L Oliveira; P B Malafaya; S A Costa; R A Sousa; R L Reis |
Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of materials science. Materials in medicine Volume: 18 ISSN: 0957-4530 ISO Abbreviation: J Mater Sci Mater Med Publication Date: 2007 Feb |
Date Detail:
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Created Date: 2007-02-26 Completed Date: 2007-06-26 Revised Date: 2008-08-14 |
Medline Journal Info:
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Nlm Unique ID: 9013087 Medline TA: J Mater Sci Mater Med Country: United States |
Other Details:
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Languages: eng Pagination: 211-23 Citation Subset: IM |
Affiliation:
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3B's Research Group-Biomaterials, Biodegradables and Biomimetics, Univ. of Minho, Campus Gualtar, 4710 Braga, Portugal. analeite@dep.uminho.pt |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apatites
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chemistry* Biomimetic Materials / chemistry* Bone Substitutes / chemistry Cell Culture Techniques / methods Coated Materials, Biocompatible / chemistry* Crystallization / methods Extracellular Matrix / chemistry Imaging, Three-Dimensional / methods* Materials Testing / methods Particle Size Polyesters / chemistry* Porosity Starch / chemistry Surface Properties Tissue Engineering / methods* Tomography, X-Ray Computed / instrumentation, methods* |
| Chemical | |
Reg. No./Substance:
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0/Apatites; 0/Bone Substitutes; 0/Coated Materials, Biocompatible; 0/Polyesters; 24980-41-4/polycaprolactone; 9005-25-8/Starch |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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