Document Detail

Micro-analysis of amino acids.
MedLine Citation:
PMID:  7153456     Owner:  NLM     Status:  MEDLINE    
An automated amino acid analyzer has been developed for the analysis of amino acids with the sensitivity at the 10-100 pmol level except for proline which requires greater than 50 pmol. omicron-Phthalaldehyde, in the presence of 2-mercaptoethanol, is used for the fluorometric detection of amino groups (Roth, M. (1971) Anal. Chem. 43, 880-882). A post-column reaction of the amino acid with sodium hypochlorite (Bohlen, P. and Mellet, M. (1979) Anal. Biochem. 94, 313-321) gives oxidation products amenable to detection with omicron-phthalaldehyde. The instrument uses high-performance liquid chromatographic pumps capable of micro-flow rates with a minimum pulsation. The method is suitable for routine analyses of amino acids at picomole levels with reproducibility and accuracy comparable to the ninhydrin-based amino acid analysis.
R A Boykins; T Y Liu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biochemical and biophysical methods     Volume:  7     ISSN:  0165-022X     ISO Abbreviation:  J. Biochem. Biophys. Methods     Publication Date:  1982 Dec 
Date Detail:
Created Date:  1983-03-11     Completed Date:  1983-03-11     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  7907378     Medline TA:  J Biochem Biophys Methods     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  55-65     Citation Subset:  IM    
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MeSH Terms
Amino Acids / analysis*
Chromatography, High Pressure Liquid / instrumentation,  methods
Indicators and Reagents
Spectrometry, Fluorescence
Reg. No./Substance:
0/Amino Acids; 0/Indicators and Reagents

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