| Micro-analysis of amino acids. | |
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MedLine Citation:
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PMID: 7153456 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An automated amino acid analyzer has been developed for the analysis of amino acids with the sensitivity at the 10-100 pmol level except for proline which requires greater than 50 pmol. omicron-Phthalaldehyde, in the presence of 2-mercaptoethanol, is used for the fluorometric detection of amino groups (Roth, M. (1971) Anal. Chem. 43, 880-882). A post-column reaction of the amino acid with sodium hypochlorite (Bohlen, P. and Mellet, M. (1979) Anal. Biochem. 94, 313-321) gives oxidation products amenable to detection with omicron-phthalaldehyde. The instrument uses high-performance liquid chromatographic pumps capable of micro-flow rates with a minimum pulsation. The method is suitable for routine analyses of amino acids at picomole levels with reproducibility and accuracy comparable to the ninhydrin-based amino acid analysis. |
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Authors:
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R A Boykins; T Y Liu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of biochemical and biophysical methods Volume: 7 ISSN: 0165-022X ISO Abbreviation: J. Biochem. Biophys. Methods Publication Date: 1982 Dec |
Date Detail:
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Created Date: 1983-03-11 Completed Date: 1983-03-11 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 7907378 Medline TA: J Biochem Biophys Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 55-65 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis* Autoanalysis Chromatography, High Pressure Liquid / instrumentation, methods Indicators and Reagents Microchemistry Spectrometry, Fluorescence |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Indicators and Reagents |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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