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Micro-sieving: isolation of whole glomeruli from a single renal needle biopsy sample.
MedLine Citation:
PMID:  20805695     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Renal biopsy samples are important not only for the diagnosis of glomerulonephritis, but also for the investigation of its pathogenesis. However, it remains difficult to biochemically analyze proteins extracted solely from the glomeruli of needle biopsy samples, since the samples contain various components like renal tubules and connective tissue. Even a recent micro-dissection method, recovering the glomeruli in the sliced sections of the biopsy samples, has not fully solved the difficulty because the amount of obtainable proteins by this method is not usually enough for protein analysis. To overcome this problem, we established a simple but reliable method to isolate whole glomeruli from needle biopsy samples. By this method, termed 'micro-sieving', we were able to isolate on average more than 50 glomeruli from a single needle biopsy sample in an hour. The amount of the extracted glomerular proteins was on average 23 μg per biopsy sample. As a representative use of this method, we were able to obtain a glomerular protein profile by fluorescent 2-dimensional electrophoresis for each of the tested patients with glomerulonephritis. 'Micro-sieving' can be used widely as a fundamental technique to analyze glomeruli in renal needle biopsy samples.
Authors:
Kenichiro Koitabashi; Kazuki Okamoto; Mitsumi Arirto; Toshiyuki Sato; Kouhei Nagai; Manae S Kurokawa; Naoya Suematsu; Takashi Yasuda; Kenjiro Kimura; Tomohiro Kato
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Publication Detail:
Type:  Journal Article     Date:  2010-08-31
Journal Detail:
Title:  Nephron. Clinical practice     Volume:  117     ISSN:  1660-2110     ISO Abbreviation:  Nephron Clin Pract     Publication Date:  2011  
Date Detail:
Created Date:  2011-03-01     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101159763     Medline TA:  Nephron Clin Pract     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  c225-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 S. Karger AG, Basel.
Affiliation:
Clinical Proteomics and Molecular Medicine, St. Marianna University Graduated School of Medicine, Kawasaki, Japan.
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