Document Detail


Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells.
MedLine Citation:
PMID:  16239327     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Current methods for identifying neoplastic cells and discerning them from their normal counterparts are often nonspecific, slow, biologically perturbing, or a combination thereof. Here, we show that single-cell micro-Raman spectroscopy averts these shortcomings and can be used to discriminate between unfixed normal human lymphocytes and transformed Jurkat and Raji lymphocyte cell lines based on their biomolecular Raman signatures. We demonstrate that single-cell Raman spectra provide a highly reproducible biomolecular fingerprint of each cell type. Characteristic peaks, mostly due to different DNA and protein concentrations, allow for discerning normal lymphocytes from transformed lymphocytes with high confidence (p << 0.05). Spectra are also compared and analyzed by principal component analysis to demonstrate that normal and transformed cells form distinct clusters that can be defined using just two principal components. The method is shown to have a sensitivity of 98.3% for cancer detection, with 97.2% of the cells being correctly classified as belonging to the normal or transformed type. These results demonstrate the potential application of confocal micro-Raman spectroscopy as a clinical tool for single cancer cell detection based on intrinsic biomolecular signatures, therefore eliminating the need for exogenous fluorescent labeling.
Authors:
James W Chan; Douglas S Taylor; Theodore Zwerdling; Stephen M Lane; Ko Ihara; Thomas Huser
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2005-10-20
Journal Detail:
Title:  Biophysical journal     Volume:  90     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-29     Completed Date:  2006-05-17     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  648-56     Citation Subset:  IM    
Affiliation:
Lawrence Livermore National Laboratory, Livermore, California 94551, USA. chan19@llnl.gov
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MeSH Terms
Descriptor/Qualifier:
B-Lymphocytes / metabolism
Biophysics / methods*
Cell Adhesion
Cell Line
Cell Line, Transformed
DNA / chemistry
Hematopoiesis
Hematopoietic Stem Cells / cytology*
Hematopoietic System
Humans
Jurkat Cells
Leukocytes, Mononuclear / metabolism
Lymphocytes / metabolism
Microscopy, Confocal
Microscopy, Fluorescence
Neoplasms / metabolism*
Principal Component Analysis
RNA / chemistry
Reproducibility of Results
Spectrophotometry
Spectrum Analysis, Raman / methods*
Time Factors
Chemical
Reg. No./Substance:
63231-63-0/RNA; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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