Document Detail


Micelle-vesicle transition of egg phosphatidylcholine and octyl glucoside.
MedLine Citation:
PMID:  3365419     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The dissolution and formation of egg phosphatidylcholine (PC) vesicles by the detergent octyl glucoside were examined systematically by using resonance energy transfer between fluorescent lipid probes, turbidity, and gel filtration chromatography. Resonance energy transfer was exquisitely sensitive to the intermolecular distance when the lipids were in the lamellar phase and to the transitions leading to mixed micelles. Turbidity measurements provided information about the aggregation of lipid and detergent. Several reversible discrete transitions between states of the PC-octyl glucoside system were observed by both methods during dissolution and vesicle formation. These states could be described as a series of equilibrium structures that took the forms of vesicles, open lamellar sheets, and mixed micelles. As detergent was added to an aqueous suspension of vesicles, the octyl glucoside partitioned into the vesicles with a partition coefficient of 63. This was accompanied by leakage of small molecules and vesicle swelling until the mole fraction of detergent in the vesicles was just under 50% (detergent:lipid ratio of 1:1). Near this point, a transition was observed by an increase in turbidity and release of large molecules like inulin, consistent with the opening of vesicles. Both a turbidity maximum and a sharp increase in fluorescence were observed at a detergent to lipid mole ratio of 2.1:1. This was interpreted as the lower boundary of a region where both lamellar sheets and micelles are at equilibrium. At a detergent:lipid ratio of 3.0:1, another sharp change in resonance energy transfer and clarification of the suspension were observed, demarcating the upper boundary of this two-phase region. This latter transition is commonly referred to as solubilization.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
M Ollivon; O Eidelman; R Blumenthal; A Walter
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemistry     Volume:  27     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1988 Mar 
Date Detail:
Created Date:  1988-06-14     Completed Date:  1988-06-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1695-703     Citation Subset:  IM    
Affiliation:
Section on Membrane Structure and Function, LTB, National Cancer Institute, Bethesda, Maryland 20892.
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MeSH Terms
Descriptor/Qualifier:
Fluorescent Dyes
Glucosides*
Glycosides*
Lectins*
Micelles
Models, Biological
Molecular Conformation
Spectrometry, Fluorescence
Grant Support
ID/Acronym/Agency:
N01-CO-65341/CO/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Glucosides; 0/Glycosides; 0/Lectins; 0/Micelles; 29836-26-8/octyl-beta-D-glucoside

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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