| Mice lacking angiotensin-converting enzyme have low blood pressure, renal pathology, and reduced male fertility. | |
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MedLine Citation:
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PMID: 8642790 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mammals produce two isozymes of angiotensin-converting enzyme (ACE). Somatic ACE plays an important role in the control of blood pressure. The function of testis ACE, produced by male and germ cells, is not known. To examine the roles of these isozymes, we used targeted homologous recombination to introduce a modified ACE allele into a mouse line. Mice homozygous for this mutant allele lack both ACE isozymes and have markedly reduced blood pressures. Contrary to a previous report, we found heterozygous male mice to have normal blood pressures. Homozygous mutant mice also have severe renal disease. The renal papilla is markedly reduced, and the intrarenal arteries exhibit vascular hyperplasia associated with a perivascular inflammatory infiltrate. These animals cannot effectively concentrate urine. They also have an abnormally low urinary sodium to potassium ratio despite reduced levels of aldosterone. Homozygous mutant male mice sire significantly smaller litters than wild-type male mice; however, no defect in sperm number, morphology, or motility was detected. ACE-deficient animals demonstrate the role of this enzyme in systemic blood pressure, renal development and function, and male fertility. |
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Authors:
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C R Esther; T E Howard; E M Marino; J M Goddard; M R Capecchi; K E Bernstein |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Laboratory investigation; a journal of technical methods and pathology Volume: 74 ISSN: 0023-6837 ISO Abbreviation: Lab. Invest. Publication Date: 1996 May |
Date Detail:
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Created Date: 1996-07-16 Completed Date: 1996-07-16 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0376617 Medline TA: Lab Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 953-65 Citation Subset: IM |
Affiliation:
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Department of Pathology, Emory University, Atlanta, Georgia 30322, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Blood Pressure* Female Homozygote Infertility, Male / etiology*, pathology, physiopathology Isoenzymes / blood, deficiency, genetics, physiology Kidney / enzymology, pathology, physiopathology Kidney Concentrating Ability Kidney Diseases / etiology*, pathology, physiopathology Male Mice Mice, Mutant Strains Molecular Sequence Data Peptidyl-Dipeptidase A / blood, deficiency, genetics, physiology* Polymerase Chain Reaction Recombination, Genetic Testis / enzymology, pathology Water Deprivation / physiology |
| Grant Support | |
ID/Acronym/Agency:
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DK39777/DK/NIDDK NIH HHS; DK44280/DK/NIDDK NIH HHS; DK45215/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; EC 3.4.15.1/Peptidyl-Dipeptidase A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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