Document Detail

Mice completely suppressed for the expression of immunoglobulin kappa light chain.
MedLine Citation:
PMID:  6420788     Owner:  NLM     Status:  MEDLINE    
Complete suppression of expression of immunoglobulin kappa light chain was achieved by injecting female mice from birth with a mixture of antisera against the mu heavy chain and kappa light chain (anti-mu and anti-kappa). Then their offspring were injected with anti-kappa from birth. This resulted in stable suppression as long as anti-kappa injections were continued. kappa light chain was not detectable either in serum or at the cellular level. The number of B cells in spleen and the concentration of immunoglobulin classes and subclasses in serum were normal. The normal levels were achieved by a compensating enhancement of lambda light chain expression. Analysis of the light chains of immunoglobulins secreted by spleen cells from suppressed mice after liposaccharide stimulation by two-dimensional gels showed lambda chain to have a limited heterogeneity. Primary responses to dinitrophenol, influenza strain A, and keyhole limpet hemocyanin were drastically affected, whereas secondary responses appeared to be quite normal, suggesting a surprisingly large potential repertoire.
S Weiss; K Lehmann; W C Raschke; M Cohn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  81     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1984 Jan 
Date Detail:
Created Date:  1984-03-01     Completed Date:  1984-03-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  211-5     Citation Subset:  IM    
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MeSH Terms
Antibody Formation*
Immune Sera
Immunoglobulin Heavy Chains / immunology
Immunoglobulin Light Chains / genetics*
Immunoglobulin kappa-Chains / genetics*,  immunology
Immunoglobulin mu-Chains / immunology
Mice, Inbred BALB C
Grant Support
A105875//PHS HHS; CA30903/CA/NCI NIH HHS
Reg. No./Substance:
0/Immune Sera; 0/Immunoglobulin Heavy Chains; 0/Immunoglobulin Light Chains; 0/Immunoglobulin kappa-Chains; 0/Immunoglobulin mu-Chains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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