Document Detail

Mice lacking two sperm serine proteases, ACR and PRSS21, are subfertile, but the mutant sperm are infertile in vitro.
MedLine Citation:
PMID:  20484738     Owner:  NLM     Status:  MEDLINE    
Although sperm serine protease and proteasome have long been believed to play an important role in the fertilization process, the molecular mechanism is still controversial. In this study, we have produced double-knockout mice lacking two sperm serine proteases, ACR and PRSS21, to uncover the functional role of the trypsinlike activity in fertilization. The double-knockout male mice were subfertile, likely owing to the incompleteness of fertilization in the oviductal ampulla. Despite male subfertility, the mutant epididymal sperm exhibited the inability to undergo acrosomal exocytosis on the zona pellucida (ZP) surface and to traverse the ZP, thus resulting in the failure of fertilization in vitro. The double-knockout epididymal sperm were also defective in penetration through the cumulus matrix to reach the ZP. When epididymal sperm were artificially injected into the uterus of wild-type mice, the 2-cell embryos, which had previously been fertilized by double-knockout sperm, were recovered at a low but significant level. The mutant epididymal sperm were also capable of fertilizing the oocytes in the presence of uterine fluids in vitro. These data demonstrate that the trypsinlike protease activity of ACR and PRSS21 is essential for the process of sperm penetration through the cumulus matrix and ZP in vitro, and suggest that the female reproductive tract partially compensates for the loss of the sperm function. We therefore conclude that the sperm trypsinlike activity is still important but not essential for fertilization in vivo in the mouse.
Natsuko Kawano; Woojin Kang; Misuzu Yamashita; Yoshitaka Koga; Taiga Yamazaki; Tamako Hata; Kenji Miyado; Tadashi Baba
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-19
Journal Detail:
Title:  Biology of reproduction     Volume:  83     ISSN:  1529-7268     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-23     Completed Date:  2010-11-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  359-69     Citation Subset:  IM    
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba Science City, Ibaraki, Japan.
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MeSH Terms
Acrosin / genetics,  metabolism*
Acrosome Reaction / physiology
Blotting, Western
Cumulus Cells / physiology
Fertility / physiology*
Fertilization / physiology*
GPI-Linked Proteins / genetics,  metabolism
Infertility, Male / genetics,  metabolism*
Mice, Inbred ICR
Mice, Knockout
Serine Endopeptidases / genetics,  metabolism*
Sperm-Ovum Interactions / physiology
Spermatozoa / physiology*
Zona Pellucida / physiology
Reg. No./Substance:
0/GPI-Linked Proteins; EC 3.4.21.-/Prss21 protein, mouse; EC 3.4.21.-/Serine Endopeptidases; EC

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