| MiR-34a inhibits lymphatic metastasis potential of mouse hepatoma cells. | |
| | |
MedLine Citation:
|
PMID: 21553024 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
MicroRNAs are small non-coding RNAs that regulate the expression of other genes in a post-transcriptional manner. MiR-34a can induce apoptosis, cell cycle arrest, and senescence. However, its role in tumor progress remains to be fully elucidated. In the present study, the role of miR-34a in lymphatic metastasis was investigated using mouse hepatocarcinoma cell lines Hca-F and Hepa1-6. MicroRNA profiling and Hairpin-RT-PCR analysis showed that the expression level of miR-34a was higher in Hepa1-6 cells (of no metastatic ability) than that in Hca-F cells (of high metastatic ability). Ectopic expression of miR-34a can inhibit cell growth and cell invasion in Hepa1-6 and Hca-F cells. Moreover, miR-34a triggers G1 arrest and down-regulates CyclinD1 and CDK6 in Hepa1-6 cells. Furthermore, we proved that miR-34a decreased adhesion of Hca-F cells to regional lymph node in vitro, reduced lymph nodes-metastasized burden, and inhibited tumor lymph node metastases in vivo. All these results suggest that miR-34a plays multiple tumor suppressive roles in murine hepatocarcinoma, not only inhibiting cell growth by cell cycle arrest, but also repressing metastasis, and may serve as a novel therapeutic target for hepatocarcinoma. |
| | |
Authors:
|
Yanjie Guo; Sheng Li; Jianhua Qu; Shujing Wang; Yibing Dang; Jianhui Fan; Shengjin Yu; Jianing Zhang |
Related Documents
:
|
19870054 - Cellular reactions in the meninges of rabbits to tuberculo-lipoid, protein, and polysac... 19025454 - Photothermal bubbles as optical scattering probes for imaging living cells. 17942794 - Development of a ligand blot assay using biotinylated live cells. 8376464 - Synchrony of cell spreading and contraction force as phagocytes engulf large pathogens. 18155194 - Presence of egf growth factor ligands and their effects on cultured rat conjunctival go... 9124594 - Parathyroid hormone-related protein, an autocrine growth inhibitor of alveolar type ii ... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-5-7 |
Journal Detail:
|
Title: Molecular and cellular biochemistry Volume: - ISSN: 1573-4919 ISO Abbreviation: - Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-5-9 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Biochemistry, Institute of Glycobiology, Dalian Medical University, 9 South Lvshun Road Western Section, Dalian, 116044, Liaoning, China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: CXCR4 positive cells from Lewis lung carcinoma cell line have cancer metastatic stem cell characteri...
Next Document: Reduced dosage of the modifiers of epigenetic reprogramming Dnmt1, Dnmt3L, SmcHD1 and Foxo3a has no ...