Document Detail


MiR-297 modulate multidrug resistance in human colorectal carcinoma by down-regulating MRP-2.
MedLine Citation:
PMID:  22676135     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Colorectal carcinoma is a frequent cause of cancer-related death in the world for men and women. MicroRNAs are endogenous small noncoding RNAs that regulate gene expression negatively at posttranscriptional level. Here we investigated the possible role of microRNAs in the development of multidrug resistance (MDR) in colorectal carcinoma cells. We analyzed miRNA expression levels between multidrug resistant colorectal carcinoma cell line HCT116/L-OHP and its parent cell line HCT116 using a miRNA microarray. MiR-297 had the lower expression of miRNA in HCT116/L-OHP cells compared to its parental cells. MRP-2 is an important MDR protein in platinum-drug resistance cells; meanwhile MRP-2 is a predicted target of miR-297. Additionally, miR-297 was downregulated in a panel of human colorectal carcinoma tissues and consistent reversely with expression levels of MRP-2. Furthermore, we found that ectopic expression of miR-297 in MDR colorectal carcinoma cells reduced MRP-2 protein level and sensitized these cells to anticancer drugs in vitro and in vivo. Taken together, our findings suggest that miR-297 could play a role in the development of MDR in colorectal carcinoma cells at least in part by modulation of MRP-2.
Authors:
Ke Xu; Xin Liang; Ke Shen; Daling Cui; Yuanhong Zheng; Jianhua Xu; Zhongze Fan; Yanyan Qiu; Qi Li; Lei Ni; Jianwen Liu
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-7
Journal Detail:
Title:  The Biochemical journal     Volume:  -     ISSN:  1470-8728     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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