Document Detail


MiR-21 regulates EMT phenotype and HIF-1α expression in third-sphereforming breast cancer stem cell-like cells.
MedLine Citation:
PMID:  22435731     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Cancer stem cells (CSCs) are predicted to be critical drivers of tumor progression due to their "stemness", but the molecular mechanism of CSCs in regulating metastasis remains to be elucidated. Epithelial-mesenchymal transition (EMT), HIF-1α, and miR-21, all of which contribute to cell migrate for metastasis, are interrelate with CSCs. In this study, third-sphereforming (3-S) CSC-like cells, which showed elevated CSC surface markers (ALDH1(+) and CD44(+) /CD24(-/low) ) and sphereforming capacity as well as migration and invasion capacities, were cultured and isolated from breast cancer MCF-7 parental cells, to evaluate the role of miR-21 in regulating the CSC-like cell biological features, especially EMT. EMT, which was assessed by over-expression of mesenchymal cell markers (N-cadherin, Vimentin, α-SMA) and suppression of epithelial cell marker (E-cadherin), was induced in 3-S CSC-like cells. Moreover, both of HIF-1α and miR-21 were up-regulated in the CSC-like cells. Interestingly, antagonism of miR-21 by antagomir led to reversal of EMT, down-expression of HIF-1α, as well as suppression of invasion and migration, which indicates a key role of miR-21 involving in regulate CSC-associated features. In conclusion, we demonstrated that the formation of CSC-like cells undergoing process of EMT-like associated with over-expression of HIF-1α, both of which are regulated by miR-21. © 2012 Japanese Cancer Association.
Authors:
Mingli Han; Yimeng Wang; Manran Liu; Xiaokai Bi; Junjie Bao; Ni Zeng; Zhikun Zhu; Zhiqiang Mo; Chengyi Wu; Xin Chen
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-21
Journal Detail:
Title:  Cancer science     Volume:  -     ISSN:  1349-7006     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101168776     Medline TA:  Cancer Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Japanese Cancer Association.
Affiliation:
Department of General Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China; The Key Laboratory of Laboratory Medical Diagnostics in the Ministry of Education and Department of Clinical Biochemistry, Chongqing Medical University, Chongqing, 400016, China.
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