Document Detail


MiR-205 determines the radioresistance of human nasopharyngeal carcinoma by directly targeting PTEN.
MedLine Citation:
PMID:  22374676     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Radiotherapy is the primary treatment for nasopharyngeal carcinoma (NPC), but radioresistance severely reduces NPC radiocurability. Here, we have established a radio-resistant NPC cell line, CNE-2R, and investigate the role of miRNAs in radioresistance. The miRNAs microarray assay reveals that miRNAs are differentially expressed between CNE-2R and its parental cell line CNE-2. We find that miR-205 is elevated in CNE-2R. A target prediction algorithm suggests that miR‑205 regulates expression of PTEN, a tumor-suppressor. Introducing miR-205 into CNE-2 cells suppresses PTEN protein expression, followed by activation of AKT, increased number of foci formation and reduction of cell apoptosis postirradiation. On the other hand, knocking down miR-205 in CNE-2R cells compromises the inhibition of PTEN and increases cell apoptosis. Significantly, immunohistochemistry studies demonstrate that PTEN is downregulated at late stages of NPC, and that miR-205 is significantly elevated followed the radiotherapy. Our data conclude that miR-205 contributes to radioresistance of NPC by directly targeting PTEN. Both miR-205 and PTEN are potential predictive biomarkers for radiosensitivity of NPC and may serve as targets for achieve successful radiotherapy in NPC.
Authors:
Changju Qu; Zhihui Liang; JiaLing Huang; Ruiying Zhao; Chunhui Su; Sumei Wang; Xudan Wang; Rong Zhang; Mong-Hong Lee; Huiling Yang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell cycle (Georgetown, Tex.)     Volume:  11     ISSN:  1551-4005     ISO Abbreviation:  Cell Cycle     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-29     Completed Date:  2012-09-14     Revised Date:  2013-09-24    
Medline Journal Info:
Nlm Unique ID:  101137841     Medline TA:  Cell Cycle     Country:  United States    
Other Details:
Languages:  eng     Pagination:  785-96     Citation Subset:  IM    
Affiliation:
Department of Pathophysiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Cell Line, Tumor
Cell Proliferation / radiation effects
Flow Cytometry
Humans
MicroRNAs / genetics,  metabolism*
Nasopharyngeal Neoplasms / metabolism*
PTEN Phosphohydrolase / genetics,  metabolism*
Radiation Tolerance / genetics*
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
CA16672/CA/NCI NIH HHS; P30 CA016672/CA/NCI NIH HHS; R01CA089266/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/MIRN205 microRNA, human; 0/MicroRNAs; EC 3.1.3.48/PTEN protein, human; EC 3.1.3.67/PTEN Phosphohydrolase
Comments/Corrections

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