Document Detail


MiR-195 regulates cell apoptosis of human hepatocellular carcinoma cells by targeting LATS2.
MedLine Citation:
PMID:  22888524     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Recently, we have reported tissue- and stage-specific expression of miR-195 in human hepatocellular carcinoma cells and so far, not many reports discuss the function of this microRNA (miRNA). Expression profiling of miRNAs revealed a limited set of miRNAs with altered expression in drug resistant hepatocellular carcinoma cell line BEL-7402/5-FU compared to its parental BEL-7402 cell line. Real-time PCR confirmed down-regulation of miR-195 in BEL-7402/5-FU cells. Western blots were performed to determine protein levels of LATS2, P53 and CDK2. MTT analysed the cell proliferation activity. Flow cytometry were performed to determine apoptosis rate. Up-regulation of miR-195 increased expression of LATS2 and increased apoptosis of HCC cells, while Anti-miR-195 treatment inhibited expression of LATS2. miR-195 over-expression inhibited the luciferase activity of a LATS2 3' untranslated region-based reporter construct in BEL-7402/5-FU cells. These results indicate that miR-195 could increase cell apoptosis by targeting LATS2 in hepatocellular carcinoma cells.
Authors:
Xiaoyan Yang; Jia Yu; Jie Yin; Qiong Xiang; Huifang Tang; Xiaoyong Lei
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Die Pharmazie     Volume:  67     ISSN:  0031-7144     ISO Abbreviation:  Pharmazie     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-08-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9800766     Medline TA:  Pharmazie     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  645-51     Citation Subset:  IM    
Affiliation:
Institute of Pharmacy and Pharmacology, The First Affilitated Hospital, University of South China Hengyang, P.R. China.
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