Document Detail


MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb.
MedLine Citation:
PMID:  17923094     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MiR-150 is a microRNA (miRNA) specifically expressed in mature lymphocytes, but not their progenitors. A top predicted target of miR-150 is c-Myb, a transcription factor controlling multiple steps of lymphocyte development. Combining loss- and gain-of-function gene targeting approaches for miR-150 with conditional and partial ablation of c-Myb, we show that miR-150 indeed controls c-Myb expression in vivo in a dose-dependent manner over a narrow range of miRNA and c-Myb concentrations and that this dramatically affects lymphocyte development and response. Our results identify a key transcription factor as a critical target of a stage-specifically expressed miRNA in lymphocytes and suggest that this and perhaps other miRNAs have evolved to control the expression of just a few critical target proteins in particular cellular contexts.
Authors:
Changchun Xiao; Dinis Pedro Calado; Gunther Galler; To-Ha Thai; Heide Christine Patterson; Jing Wang; Nikolaus Rajewsky; Timothy P Bender; Klaus Rajewsky
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Cell     Volume:  131     ISSN:  0092-8674     ISO Abbreviation:  Cell     Publication Date:  2007 Oct 
Date Detail:
Created Date:  2007-10-09     Completed Date:  2008-02-21     Revised Date:  2014-09-12    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  146-59     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
3' Untranslated Regions
Animals
B-Lymphocytes / cytology,  physiology*
Cell Death
Cell Differentiation / physiology*
Cells, Cultured
Gene Expression Regulation*
Gene Targeting
Genes, Reporter
Humans
Immune System / physiology
Mice
Mice, Knockout
MicroRNAs / genetics,  metabolism*
Proto-Oncogene Proteins c-myb / genetics,  metabolism*
T-Lymphocytes / physiology
Grant Support
ID/Acronym/Agency:
AI054636/AI/NIAID NIH HHS; AI059294/AI/NIAID NIH HHS; AI064345/AI/NIAID NIH HHS; CA85842/CA/NCI NIH HHS; R01 AI059294/AI/NIAID NIH HHS; R01 CA085842/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/3' Untranslated Regions; 0/MicroRNAs; 0/Proto-Oncogene Proteins c-myb
Comments/Corrections
Comment In:
Cell. 2007 Oct 5;131(1):22-4   [PMID:  17923083 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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