Document Detail


MgAtg9 trafficking in Magnaporthe oryzae.
MedLine Citation:
PMID:  19556868     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Autophagy is a vacuolar/lysosomal cytoplasmic recycling system in eukaryotic cells. ScATG9 is indispensable for autophagy in Saccharomyces cerevisiae. Here, we deleted MgATG9, the orthologue of ScATG9, via targeted gene replacement in the phytopathogenic filamentous fungus Magnaporthe oryzae, and then analyzed the cellular distribution pattern of EGFP-MgAtg9 in the Mgatg9Delta mutant. We detected an expression profile of multiple green dots in the conidial cell inoculated in rich media and in the appressoria differentiated from the conidia in H(2)O. Concurrent with the punctation, we found some fluorescent signals localized on the central vacuole of the submerged hyphae from the conidia cultured in rich media. Next, we introduced DsRed2-MgAtg8 into the Mgatg9Delta mutant expressing EGFP-MgAtg9 and observed partial overlap at multiple sites in the conidial cell, reminiscent of that in the mammalian system. Our findings further led to the postulation that the multiple sites where the two fusions colocalized tend to merge as a central structure in the conidial cell. Finally, we tested the expression of EGFP-MgAtg9 in null mutants of MgATG1, 2, 13 and 18, respectively. We speculate that MgAtg1, 2 and 18, but not MgAtg13, is required for MgAtg9 cycling through the multiple colocalization sites to its storage pools in the conidial cell of M. oryzae, and fusion of these colocalization sites into a central structure could be governed through other unidentified mechanisms.
Authors:
Bo Dong; Xiao-Hong Liu; Jian-Ping Lu; Fu-Sheng Zhang; Hui-Min Gao; Hong-Kai Wang; Fu-Cheng Lin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-03
Journal Detail:
Title:  Autophagy     Volume:  5     ISSN:  1554-8635     ISO Abbreviation:  Autophagy     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-19     Completed Date:  2010-01-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101265188     Medline TA:  Autophagy     Country:  United States    
Other Details:
Languages:  eng     Pagination:  946-53     Citation Subset:  IM    
Affiliation:
State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Autophagy / physiology*
Fungal Proteins / genetics,  metabolism*
Green Fluorescent Proteins / genetics,  metabolism
Hyphae / metabolism,  ultrastructure
Magnaporthe / cytology,  metabolism*
Membrane Proteins / genetics,  metabolism*
Mutagenesis
Protein Isoforms / genetics,  metabolism
Recombinant Fusion Proteins / genetics,  metabolism
Saccharomyces cerevisiae Proteins / genetics,  metabolism*
Vacuoles / metabolism,  ultrastructure
Chemical
Reg. No./Substance:
0/ATG9 protein, S cerevisiae; 0/Fungal Proteins; 0/Membrane Proteins; 0/Protein Isoforms; 0/Recombinant Fusion Proteins; 0/Saccharomyces cerevisiae Proteins; 147336-22-9/Green Fluorescent Proteins

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