Document Detail


The MexJK efflux pump of Pseudomonas aeruginosa requires OprM for antibiotic efflux but not for efflux of triclosan.
MedLine Citation:
PMID:  12193619     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using the biocide triclosan as a selective agent, several triclosan-resistant mutants of a susceptible Pseudomonas aeruginosa strain were isolated. Cloning and characterization of a DNA fragment conferring triclosan resistance from one of these mutants revealed a hitherto uncharacterized efflux system of the resistance nodulation cell division (RND) family, which was named MexJK and which is encoded by the mexJK operon. Expression of this operon is negatively regulated by the product of mexL, a gene located upstream of and transcribed divergently from mexJK. The triclosan-resistant mutant contained a single nucleotide change in mexL, which caused an amino acid change in the putative helix-turn-helix domain of MexL. The MexL protein belongs to the TetR family of repressor proteins. The MexJK system effluxed tetracycline and erythromycin but only in the presence of the outer membrane protein channel OprM; OprJ and OprN did not function with MexJK. Triclosan efflux required neither of the outer membrane protein channels tested but necessitated the MexJ membrane fusion protein and the MexK inner membrane RND transporter. The results presented in this study suggest that MexJK may function as a two-component RND pump for triclosan efflux but must associate with OprM to form a tripartite antibiotic efflux system. Furthermore, the results confirm that triclosan is an excellent tool for the study of RND multidrug efflux systems and that this popular biocide therefore readily selects mutants which are cross-resistant with antibiotics.
Authors:
Rungtip Chuanchuen; Craig T Narasaki; Herbert P Schweizer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  184     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-08-23     Completed Date:  2002-10-04     Revised Date:  2013-06-09    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5036-44     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado 80523-1682, USA.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Anti-Bacterial Agents / metabolism*,  pharmacology
Anti-Infective Agents, Local / metabolism*,  pharmacology
Bacterial Outer Membrane Proteins / genetics,  metabolism*
Bacterial Proteins / chemistry,  genetics*,  metabolism
Base Sequence
Biological Transport
Carrier Proteins / genetics,  metabolism*
Cloning, Molecular
Drug Resistance, Bacterial / genetics
Gene Expression Regulation, Bacterial
Membrane Transport Proteins / chemistry,  genetics*,  metabolism*
Microbial Sensitivity Tests
Molecular Sequence Data
Pseudomonas aeruginosa / drug effects*,  genetics
Sequence Analysis, DNA
Triclosan / metabolism*,  pharmacology
Grant Support
ID/Acronym/Agency:
GM56685/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Anti-Infective Agents, Local; 0/Bacterial Outer Membrane Proteins; 0/Bacterial Proteins; 0/Carrier Proteins; 0/Membrane Transport Proteins; 0/OprM protein, Pseudomonas aeruginosa; 3380-34-5/Triclosan
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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