Document Detail


Methyltransferases mediate cell memory of a genotoxic insult.
MedLine Citation:
PMID:  21057543     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Characterization of the direct effects of DNA-damaging agents shows how DNA lesions lead to specific mutations. Yet, serum from Hiroshima survivors, Chernobyl liquidators and radiotherapy patients can induce a clastogenic effect on naive cells, showing indirect induction of genomic instability that persists years after exposure. Such indirect effects are not restricted to ionizing radiation, as chemical genotoxins also induce heritable and transmissible genomic instability phenotypes. Although such indirect induction of genomic instability is well described, the underlying mechanism has remained enigmatic. Here, we show that mouse embryonic stem cells exposed to γ-radiation bear the effects of the insult for weeks. Specifically, conditioned media from the progeny of exposed cells can induce DNA damage and homologous recombination in naive cells. Notably, cells exposed to conditioned media also elicit a genome-destabilizing effect on their neighbouring cells, thus demonstrating transmission of genomic instability. Moreover, we show that the underlying basis for the memory of an insult is completely dependent on two of the major DNA cytosine methyltransferases, Dnmt1 and Dnmt3a. Targeted disruption of these genes in exposed cells completely eliminates transmission of genomic instability. Furthermore, transient inactivation of Dnmt1, using a tet-suppressible allele, clears the memory of the insult, thus protecting neighbouring cells from indirect induction of genomic instability. We have thus demonstrated that a single exposure can lead to long-term, genome-destabilizing effects that spread from cell to cell, and we provide a specific molecular mechanism for these persistent bystander effects. Collectively, our results impact the current understanding of risks from toxin exposures and suggest modes of intervention for suppressing genomic instability in people exposed to carcinogenic genotoxins.
Authors:
R E Rugo; J T Mutamba; K N Mohan; T Yee; J R Chaillet; J S Greenberger; B P Engelward
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-11-08
Journal Detail:
Title:  Oncogene     Volume:  30     ISSN:  1476-5594     ISO Abbreviation:  Oncogene     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-02-10     Completed Date:  2011-04-08     Revised Date:  2013-12-06    
Medline Journal Info:
Nlm Unique ID:  8711562     Medline TA:  Oncogene     Country:  England    
Other Details:
Languages:  eng     Pagination:  751-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bystander Effect / genetics*
Cells, Cultured
Culture Media, Conditioned / chemistry,  toxicity
DNA (Cytosine-5-)-Methyltransferase / genetics,  metabolism*
DNA Damage*
Embryonic Stem Cells / drug effects,  enzymology*,  radiation effects*
Gamma Rays
Genomic Instability*
Mice
Grant Support
ID/Acronym/Agency:
P30-ES002109/ES/NIEHS NIH HHS; R01 CA083876-08/CA/NCI NIH HHS; R01 HD044133/HD/NICHD NIH HHS; R01-CA83876-8/CA/NCI NIH HHS; U19 AI068021/AI/NIAID NIH HHS; U19 AI068021-05/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; EC 2.1.1.37/DNA (Cytosine-5-)-Methyltransferase; EC 2.1.1.37/DNA (cytosine-5-)-methyltransferase 1; EC 2.1.1.37/DNA methyltransferase 3A
Comments/Corrections

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