Document Detail


The methyltransferase activity of Clr4Suv39h triggers RNAi independently of histone H3K9 methylation.
MedLine Citation:
PMID:  20705239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In fission yeast, the pericentromeric dg and dh repeats are transcribed and give rise to small interfering RNAs (siRNAs) by a mechanism that depends on the Clr4(suv39h) histone H3 lysine 9 (H3K9) methyltransferase. Here, we show that Clr4 activity promotes the assembly of a tripartite complex composed of the Clr4-containing CLRC complex and complexes involved in siRNA generation. However, unlike dh siRNAs, dg siRNAs accumulate to near wild-type levels in cells with H3K9 substitutions that cannot be methylated. Thus, Clr4 activity controls siRNA amplification from the different repeat regions by different mechanisms, H3K9 methylation dependent versus independent. Furthermore, artificial tethering of Rik1, a core subunit of the CLRC complex, to a euchromatic RNA mediates RNAi-dependent silencing that partially bypasses the requirement for other CLRC subunits. These findings establish Rik1 as a key link between CLRC and RNAi and reveal distinct centromeric siRNA amplification mechanisms that depend on the Clr4 methyltransferase activity.
Authors:
Erica L Gerace; Mario Halic; Danesh Moazed
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular cell     Volume:  39     ISSN:  1097-4164     ISO Abbreviation:  Mol. Cell     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-13     Completed Date:  2010-09-10     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  9802571     Medline TA:  Mol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  360-72     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle Proteins / genetics,  metabolism*
Centromere / genetics,  metabolism
Chromosomal Proteins, Non-Histone / genetics,  metabolism
Gene Silencing / physiology
Histones / genetics,  metabolism*
Methylation
Methyltransferases / genetics,  metabolism*
Multienzyme Complexes / genetics,  metabolism*
RNA, Fungal / genetics,  metabolism*
RNA, Small Interfering / genetics,  metabolism*
Schizosaccharomyces / enzymology*,  genetics
Schizosaccharomyces pombe Proteins / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
GM72805/GM/NIGMS NIH HHS; R01 GM061641/GM/NIGMS NIH HHS; R01 GM061641-07/GM/NIGMS NIH HHS; R01 GM072805/GM/NIGMS NIH HHS; //Howard Hughes Medical Institute; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Chromosomal Proteins, Non-Histone; 0/Histones; 0/Multienzyme Complexes; 0/RNA, Fungal; 0/RNA, Small Interfering; 0/Rik1 protein, S pombe; 0/Schizosaccharomyces pombe Proteins; 0/clr4 protein, S pombe; EC 2.1.1.-/Methyltransferases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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