| Methylthiohydantoin amino acids: chromatographic separation and comparison to phenylthiohydantoin amino acids. | |
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MedLine Citation:
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PMID: 541446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Most phenylthiohydantoin (PTH) amino acids and most methylthiohydantoin (MTH) amino acids may be separated from one another by thin-layer chromatography (TLC) using the same sequential development technique with the same two solvents. Similarly, a single solvent system may be used in high-performance liquid chromatography (HPLC) to separate most PTH-amino acids and most MTH-amino acids. When both TLC and HPLC separations are performed on a sample, all MTH-and PTH-amino acids can be uniquely identified. Since many solid-phase protein sequencing techniques generate both MTH-and PTH-amino acids, these analytical systems simplify identification of the amino acid derivatives. Although the chromatographic properties of MTH-and PTH-amino acids are similar, they are not identical (contrary to a previous report). |
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Authors:
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M J Horn; P A Hargrave; J K Wang |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of chromatography Volume: 180 ISSN: 0021-9673 ISO Abbreviation: J. Chromatogr. Publication Date: 1979 Nov |
Date Detail:
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Created Date: 1980-06-16 Completed Date: 1980-06-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0427043 Medline TA: J Chromatogr Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 111-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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isolation & purification* Chromatography, High Pressure Liquid / methods Chromatography, Thin Layer / methods Hydantoins* Indicators and Reagents Peptides* Phenylthiohydantoin* Proteins* Thiohydantoins* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Hydantoins; 0/Indicators and Reagents; 0/Peptides; 0/Proteins; 0/Thiohydantoins; 2010-15-3/Phenylthiohydantoin; 694-68-8/methylthiohydantoin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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