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Methylthioadenosine phosphorylase inactivation depends on gene deletion in laryngeal squamous cell carcinoma.
MedLine Citation:
PMID:  23020581     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Conde L, Vilaseca I, Alós L, Bernal-Sprekelsen M, Cardesa A & Nadal A (2012) Histopathology Methylthioadenosine phosphorylase inactivation depends on gene deletion in laryngeal squamous cell carcinoma Aims:  Methylthioadenosine phosphorylase (MTAP) is an essential enzyme for the methionine and adenosine salvage pathway in normal cells, frequently inactivated in many different human cancers. MTAP status could be important for tumour cell sensitivity to adjuvant chemotherapy. To our knowledge, there have been no reports to date on MTAP status in laryngeal carcinoma. Methods and results:  A series of 31 laryngeal squamous cell carcinomas was investigated for MTAP mRNA expression using reverse transcription and quantitative polymerase chain reaction (qPCR), as well as for MTAP gene deletion and/or promoter hypermethylation using qPCR and methylation-specific PCR, respectively. Low MTAP mRNA expression was found in 32% of cases, and was associated with MTAP gene deletion (in 70%; P < 0.001) but not with MTAP promoter hypermethylation, indicating that, in this tumour, gene deletion is the main mechanism for MTAP inactivation. Neither low mRNA expression nor gene deletion was associated with any of the clinicopathological parameters investigated. Conclusion:  Given the significance of MTAP status for cell sensitivity to different chemotherapeutic regimens, our results suggest that determination of MTAP inactivation should be taken into consideration in managing laryngeal squamous cell carcinomas.
Authors:
Laura Conde; Isabel Vilaseca; Llucia Alós; Manuel Bernal-Sprekelsen; Antonio Cardesa; Alfons Nadal
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-20
Journal Detail:
Title:  Histopathology     Volume:  -     ISSN:  1365-2559     ISO Abbreviation:  Histopathology     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-10-1     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7704136     Medline TA:  Histopathology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 Blackwell Publishing Limited.
Affiliation:
Fundació Clínic per a la Recerca Biomèdica, Barcelona, Spain Servei d'Otorinolaringologia, Hospital Clínic, Universitat de Barcelona (UB), Barcelona CIBERES, Bunyola, Spain Servei d'Anatomia Patològica, Hospital Clinic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona (UB), Barcelona, Spain.
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