| Methylprednisolone and interleukin-10 reduce gray matter damage in the contused Fischer rat thoracic spinal cord but do not improve functional outcome. | |
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MedLine Citation:
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PMID: 12042099 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The effects of two antiinflammatory and neuroprotective agents, methylprednisolone (MP) and interleukin-10 (IL-10), singly and in combination on tissue damage, axonal preservation and functional recovery were studied in the contused adult Fischer rat thoracic spinal cord 12 weeks after injury. MP (30 mg/kg at 5 min, and 2 and 4 h after injury) was administered intravenously and IL-10 (15 or 30 microg/kg at 30 min after injury), intraperitoneally. MP, IL-10, or the combination significantly reduced the volume of damaged tissue (including cavities) compared to control animals. The loss of spinal tissue (cavities) was reduced after treatment with MP alone or combined with IL-10, but not with IL-10 alone. The reduction in tissue damage was confined to spinal gray matter; at the level of the lesion epicenter, the thickness of the lateral white matter columns was similar in all groups. Retrograde tracing using fast blue revealed that the number of spared propriospinal and supraspinal projections was similar in all groups at 12 weeks after the contusion. The open-field BBB-test showed no significant difference in hindlimb locomotion between groups. Our results demonstrate that all tested antiinflammatory treatments significantly increase the volume of spared spinal gray matter 3 months after a moderate contusion of the Fischer rat thoracic spinal cord, but none of the treatments improved axonal preservation or functional recovery. |
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Authors:
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Toshihiro Takami; Martin Oudega; John R Bethea; Patrick M Wood; Naomi Kleitman; Mary Bartlett Bunge |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of neurotrauma Volume: 19 ISSN: 0897-7151 ISO Abbreviation: J. Neurotrauma Publication Date: 2002 May |
Date Detail:
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Created Date: 2002-06-03 Completed Date: 2002-07-24 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8811626 Medline TA: J Neurotrauma Country: United States |
Other Details:
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Languages: eng Pagination: 653-66 Citation Subset: IM |
Affiliation:
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The Chambers Family Laboratory of Electron Microscopy, The Miami Project to Cure Paralysis, Miami, Florida, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Drug Therapy, Combination Female Hindlimb Interleukin-10 / pharmacology* Methylprednisolone / pharmacology* Movement Nerve Fibers / pathology Neuroprotective Agents / pharmacology* Rats Rats, Inbred F344 Recovery of Function / drug effects* Spinal Cord Injuries / drug therapy*, immunology, pathology |
| Grant Support | |
ID/Acronym/Agency:
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P01NS38665/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Neuroprotective Agents; 130068-27-8/Interleukin-10; 83-43-2/Methylprednisolone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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