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Methylglyoxal induces mitochondria-dependent apoptosis in sarcoma.
MedLine Citation:
PMID:  22098242     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
In the preceding paper (A. Ghosh et al. (2011) Biochemistry (Moscow), 76, 1051-1060), using several comparable tissue materials, it has been convincingly demonstrated that methylglyoxal, a normal metabolite, inhibits mitochondrial complex I of specifically malignant cells. This suggests a distinct alteration of complex I, a highly important enzyme for energy (ATP) production, in malignancy. The present paper shows that as a consequence of this inhibition mitochondrial membrane potential is drastically reduced in sarcoma tissue but not in normal skeletal muscle. This was estimated spectrofluorimetrically using the dye rhodamine 123. As a consequence, cytochrome c was released from the sarcoma mitochondria as evidenced by Western blot analysis. Moreover, on treatment with methylglyoxal membrane potential collapse of sarcoma 180 cells was also indicated by fluorescence-activated cell sorter analysis. Atomic force microscopic study demonstrated gross structural alteration specifically of tumor mitochondria on methylglyoxal treatment. All these studies suggest that methylglyoxal might initiate an apoptotic event in malignant cells.
Authors:
A Ghosh; S Bera; S Ray; T Banerjee; M Ray
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemistry. Biokhimii͡a     Volume:  76     ISSN:  1608-3040     ISO Abbreviation:  Biochemistry Mosc.     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-11-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376536     Medline TA:  Biochemistry (Mosc)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1164-71     Citation Subset:  IM    
Affiliation:
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata, 700032, India. bcmr@iacs.res.in.
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