Document Detail


Methylglyoxal induces cellular damage by increasing argpyrimidine accumulation and oxidative DNA damage in human lens epithelial cells.
MedLine Citation:
PMID:  19913507     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Methylglyoxal (MGO) is a cytotoxic metabolite and modifies tissue proteins through the Maillard reaction, resulting in advanced glycation end products (AGEs), which can alter protein structure and functions. Several MGO-derived AGEs have been described, including argpyrimidine, a fluorescent product of the MGO reaction with arginine residues. Herein, we evaluated the cytotoxic role of MGO in human lens epithelial cell line (HLE-B3). HLE-B3 cells were exposed to 400 microM MGO in the present or absence of pyridoxamine for 24h. We then examined the formation of argpyrimidine, apoptosis and oxidative stress in HLE-B3 cells. In MGO-treated HLE-B3 cells, the accumulation of argpyrimidine was markedly increased, and caspase-3 and 8-hydroxydeoxyguanosine (8-OHdG) were highly expressed, which paralleled apoptotic cell death. However, pyridoxamine (AGEs inhibitor) prevented the argpyrimidine formation and apoptosis of MGO-treated HLE-B3 cells. These results suggested that the accumulation of argpyrimidine and oxidative DNA damage caused by MGO are involved in apoptosis of HLE-B3 cells.
Authors:
Junghyun Kim; Nan Hee Kim; Eunjin Sohn; Chan-Sik Kim; Jin Sook Kim
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-12
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  391     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-27     Completed Date:  2010-03-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  346-51     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Inc. All rights reserved.
Affiliation:
Diabetic Complications Research Center, Division of Traditional Korean Medicine Integrated Research, Korea Institute of Oriental Medicine, Daejeon, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis
Caspase 3 / biosynthesis
Cell Line
DNA Damage*
Deoxyguanosine / analogs & derivatives,  biosynthesis
Epithelial Cells / drug effects,  metabolism
Glycosylation End Products, Advanced / metabolism*
Humans
Lens, Crystalline / cytology,  drug effects*,  metabolism
Ornithine / analogs & derivatives*,  metabolism
Oxidative Stress*
Pyridoxamine / pharmacology
Pyrimidines / metabolism*
Pyruvaldehyde / pharmacology*
Chemical
Reg. No./Substance:
0/8-hydroxy-2'-deoxyguanosine; 0/Glycosylation End Products, Advanced; 0/Pyrimidines; 0/argpyrimidine; 7006-33-9/Ornithine; 78-98-8/Pyruvaldehyde; 85-87-0/Pyridoxamine; 961-07-9/Deoxyguanosine; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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