Document Detail


Methylenedioxy- and Ethylenedioxy-Fused Indolocarbazoles. Potent Human Topoisomerase I Inhibitors and Antitumor Agents.
MedLine Citation:
PMID:  22339065     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The indolo[2,3-a]carbazole alkaloids constitute an important class of natural products with interesting and diverse biological activities. A series of novel ring-fused indolocarbazoles were synthesized and evaluated for inhibition of topoisomerase I-mediated relaxation of supercoiled DNA and in vitro antitumor activity. The derivatives bearing a methylenedioxy or an ethylenedioxy ring the fused onto the nonglycosylated indole (1a, 1b) demonstrated more potent anti-topoisomerase I activity. The isopropylenedioxy analogue 1c was approximately half as active as 1a, while the O-dimethoxy analogue 1d and the regioisomers 2a and 2b were essentially devoid of measurable activity, implying that the stacking with the intact DNA strand has been impeded by these compounds due to steric hindrance. The newly synthesized indolocarbazoles were screened against the NCI's 60 tumor cell lines. The order of activity, based on the mean GI50 values, is as follows: 1a > 2a ~ 1d > 1b > MCR-47 > 2b. Though in general the analogues that showed potent activity against topoisomerase I (1a, 1b) also showed potent in vitro inhibition of tumor cell growth, the antitumor activity of the anti-topoisomerase I inactive 1d and 2a were intriguing. COMPARE analyses confirmed that the topoisomerase I is the primary target for 1a and 1b; however, other target(s) or pathway(s) may also be involved, with PLD1 and MERTK suggested. Further investigation of these molecular targets against these indolocarbazoles is warranted.
Authors:
David E Zembower; Yongping Xie; Ali Koohang; Mary J Kuffel; Matthew M Ames; Yasheen Zhou; Rama Mishra; Aye Aye Mar; Michael T Flavin; Ze-Qi Xu
Related Documents :
11488965 - Molecular approaches to the taxonomic position of peruvian potato cyst nematodes and ge...
3574475 - Dna fingerprinting in birds.
9175015 - Practical role of genetic profiling and preservation stock of human tumour xenograft li...
21567745 - Problem-solving test: real-time polymerase chain reaction.
9377795 - Apoptotic dna fragmentation, and its in vitro prevention by nicotinamide, in lymphocyte...
8347625 - Synthesis and damage specificity of a novel probe for the detection of abasic sites in ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-17
Journal Detail:
Title:  Anti-cancer agents in medicinal chemistry     Volume:  -     ISSN:  1875-5992     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101265649     Medline TA:  Anticancer Agents Med Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Advanced Life Sciences, 1440 Davey Road, Woodridge, Illinois 60517, USA. zxu@advancedlifesciences.com.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Fig latex (Ficus carica L. cultivar Dottato) in combination with UV irradiation decreases the viabil...
Next Document:  Doxorubicin-Loaded Nanoparticles: New Advances in Breast Cancer Therapy.