| Methylation of cyclin-dependent kinase inhibitors, XAF1, JUNB, CDH13 and soluble Wnt inhibitors in essential thrombocythaemia. | |
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MedLine Citation:
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PMID: 20364027 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Methylation of genes regulating cell-cycle check-point (INK4 cyclin-dependent kinase inhibitors), apoptosis (XAF1), adhesion (CDH13), JUNB and Wnt signalling (soluble Wnt inhibitors) has been implicated in pathogenesis of haematological and epithelial cancers. METHOD: The authors studied the methylation status of CDKN2A, CDKN2B, XAF1, CDH13, JUNB and a panel of soluble Wnt inhibitors including WIF1, DKK3, APC, SFRP1, SFRP2, SFRP4 and SFRP5 by methylation-specific PCR in 31 bone marrow and 21 peripheral blood samples of patients with essential thrombocythaemia. RESULTS AND DISCUSSION: There was no evidence of hypermethylation of all these genes in both the BM and PB samples. Therefore, in contrast to myeloid leukaemias, methylation of these genes regulating the cell cycle, apoptosis, adhesion and Wnt signalling does not play an important role in the pathogenesis of myeloproliferative diseases. Whether differential methylation may occur in the progenitor or mature blood cell compartments remains to be verified. Our study contributes to the literature on methylation in chronic myeloproliferatve diseases. |
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Authors:
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C S Chim; T K Fung; R Liang |
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Publication Detail:
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Type: Journal Article Date: 2010-04-03 |
Journal Detail:
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Title: Journal of clinical pathology Volume: 63 ISSN: 1472-4146 ISO Abbreviation: J. Clin. Pathol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-25 Completed Date: 2011-01-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376601 Medline TA: J Clin Pathol Country: England |
Other Details:
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Languages: eng Pagination: 518-21 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine, Queen Mary Hospital, University of Hong Kong, Hong Kong. jcschim@hku.hk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Aged, 80 and over Base Sequence Bone Marrow / metabolism Cadherins / genetics, metabolism Cyclin-Dependent Kinase Inhibitor Proteins / genetics*, metabolism DNA Methylation* Female Humans Intercellular Signaling Peptides and Proteins / genetics*, metabolism Intracellular Signaling Peptides and Proteins / genetics*, metabolism Male Middle Aged Molecular Sequence Data Neoplasm Proteins / genetics, metabolism Polymerase Chain Reaction / methods Proto-Oncogene Proteins c-jun / genetics, metabolism Sequence Alignment Signal Transduction / physiology Thrombocythemia, Essential / genetics*, metabolism Wnt Proteins / physiology |
| Chemical | |
Reg. No./Substance:
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0/Cadherins; 0/Cyclin-Dependent Kinase Inhibitor Proteins; 0/H-cadherin; 0/Intercellular Signaling Peptides and Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/Neoplasm Proteins; 0/Proto-Oncogene Proteins c-jun; 0/Wnt Proteins; 0/XAF1 protein, human |
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