Document Detail

Methyl jasmonate and yeast elicitor induce differential transcriptional and metabolic re-programming in cell suspension cultures of the model legume Medicago truncatula.
MedLine Citation:
PMID:  15605242     Owner:  NLM     Status:  MEDLINE    
Exposure of cell suspension cultures of Medicago truncatula Gaerth. to methyl jasmonate (MeJA) resulted in up to 50-fold induction of transcripts encoding the key triterpene biosynthetic enzyme beta-amyrin synthase (betaAS; EC 5.4.99.-). Transcripts reached maximum levels at 24 h post-elicitation with 0.5 mM MeJA. The entry point enzymes into the phenylpropanoid and flavonoid pathways, L: -phenylalanine ammonia-lyase (PAL; EC and chalcone synthase (CHS; EC, respectively, were not induced by MeJA. In contrast, exposure of cells to yeast elicitor (YE) resulted in up to 45- and 14-fold induction of PAL and CHS transcripts, respectively, at only 2 h post-elicitation. betaAS transcripts were weakly induced at 12 h after exposure to YE. Over 30 different triterpene saponins were identified in the cultures, many of which were strongly induced by MeJA, but not by YE. In contrast, cinnamic acids, benzoic acids and isoflavone-derived compounds accumulated following exposure of cultures to YE, but few changes in phenylpropanoid levels were observed in response to MeJA. DNA microarray analysis confirmed the strong differential transcriptional re-programming of the cell cultures for multiple genes in the phenylpropanoid and triterpene pathways in response to MeJA and YE, and indicated different responses of individual members of gene families. This work establishes Medicago cell cultures as an excellent model for future genomics approaches to understand the regulation of legume secondary metabolism.
Hideyuki Suzuki; M S Srinivasa Reddy; Marina Naoumkina; Naveed Aziz; Gregory D May; David V Huhman; Lloyd W Sumner; Jack W Blount; Pedro Mendes; Richard A Dixon
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2004-12-17
Journal Detail:
Title:  Planta     Volume:  220     ISSN:  0032-0935     ISO Abbreviation:  Planta     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-14     Completed Date:  2005-05-03     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  1250576     Medline TA:  Planta     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  696-707     Citation Subset:  IM    
Plant Biology Division, Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
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MeSH Terms
Abscisic Acid / pharmacology
Acetic Acids / pharmacology*
Acyltransferases / biosynthesis
Cells, Cultured
Cyclopentanes / pharmacology*
Gene Expression Regulation, Plant / drug effects
Intramolecular Transferases / biosynthesis
Medicago truncatula / drug effects*,  genetics,  metabolism*
Oligonucleotide Array Sequence Analysis
Phenylalanine Ammonia-Lyase / biosynthesis
Plant Growth Regulators / pharmacology*
Saccharomyces cerevisiae
Salicylic Acid / pharmacology
Saponins / biosynthesis
Time Factors
Transcription, Genetic / drug effects
Reg. No./Substance:
0/Acetic Acids; 0/Cyclopentanes; 0/Oxylipins; 0/Plant Growth Regulators; 0/Saponins; 1211-29-6/methyl jasmonate; 21293-29-8/Abscisic Acid; 69-72-7/Salicylic Acid; EC 2.3.-/Acyltransferases; EC synthetase; EC Ammonia-Lyase; EC 5.4.-/Intramolecular Transferases; EC 5.4.99.-/2,3-oxidosqualene-beta-amyrin-cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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