Document Detail


Methyl jasmonate induces production of reactive oxygen species and alterations in mitochondrial dynamics that precede photosynthetic dysfunction and subsequent cell death.
MedLine Citation:
PMID:  18535010     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Methyl jasmonate (MeJa) is a well-known plant stress hormone. Upon exposure to stress, MeJa is produced and causes activation of programmed cell death (PCD) and defense mechanisms in plants. However, the early events and the signaling mechanisms of MeJa-induced cell death have yet to be fully elucidated. To obtain some insights into the early events of this cell death process, we investigated mitochondrial dynamics, chloroplast morphology and function, production and localization of reactive oxygen species (ROS) at the single-cell level as well as photosynthetic capacity at the whole-seedling level under MeJa stimulation. Our results demonstrated that MeJa induction of ROS production, which first occurred in mitochondria after 1 h of MeJa treatment and subsequently in chloroplasts by 3 h of treatment, caused a series of alterations in mitochondrial dynamics including the cessation of mitochondrial movement, the loss of mitochondrial transmembrane potential (MPT), and the morphological transition and aberrant distribution of mitochondria. Thereafter, photochemical efficiency dramatically declined before obvious distortion in chloroplast morphology, which is prior to MeJa-induced cell death in protoplasts or intact seedlings. Moreover, treatment of protoplasts with ascorbic acid or catalase prevented ROS production, organelle change, photosynthetic dysfunction and subsequent cell death. The permeability transition pore inhibitor cyclosporin A gave significant protection against MPT loss, mitochondrial swelling and subsequent cell death. These results suggested that MeJa induces ROS production and alterations of mitochondrial dynamics as well as subsequent photosynthetic collapse, which occur upstream of cell death and are necessary components of the cell death process.
Authors:
Lingrui Zhang; Da Xing
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-05
Journal Detail:
Title:  Plant & cell physiology     Volume:  49     ISSN:  1471-9053     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-14     Completed Date:  2008-08-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1092-111     Citation Subset:  IM    
Affiliation:
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University, Guangzhou 510631, PR China.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acids / pharmacology*
Antioxidants / pharmacology
Arabidopsis / cytology*,  drug effects,  metabolism*
Cell Death / drug effects
Cell Survival / drug effects
Chloroplasts / drug effects,  metabolism
Cyclopentanes / pharmacology*
Cyclosporine / pharmacology
Fluorescence
Green Fluorescent Proteins / metabolism
Membrane Potential, Mitochondrial / drug effects
Mitochondria / drug effects*,  metabolism*
Mitochondrial Swelling / drug effects
Oryza sativa / drug effects,  metabolism
Oxylipins / pharmacology*
Photosynthesis / drug effects*
Protoplasts / cytology,  drug effects,  metabolism
Reactive Oxygen Species / metabolism*
Seedling / drug effects,  metabolism
Subcellular Fractions / drug effects
Time Factors
Vicia faba / drug effects,  metabolism
Chemical
Reg. No./Substance:
0/Acetic Acids; 0/Antioxidants; 0/Cyclopentanes; 0/Oxylipins; 0/Reactive Oxygen Species; 1211-29-6/methyl jasmonate; 147336-22-9/Green Fluorescent Proteins; 59865-13-3/Cyclosporine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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