Document Detail

Methyl-detected 'out-and-back' NMR experiments for simultaneous assignments of Alabeta and Ilegamma2 methyl groups in large proteins.
MedLine Citation:
PMID:  19274445     Owner:  NLM     Status:  MEDLINE    
A set of sensitive methyl-detected 'out-and-back' NMR experiments for simultaneous assignments of Alabeta and Ilegamma2 methyl positions in large proteins is described. The developed methodology is applied to an 82-kDa enzyme Malate Synthase G. Complete alanine beta and isoleucine gamma2 1H-13C methyl chemical shift assignments could be obtained from the set of new methyl-detected 'out-and-back' 3D experiments. The described methodology for methyl assignments should be applicable to protein molecules within approximately 100-kDa molecular weight range irrespective of the labeling strategy chosen to produce selectively protonated Alabeta and Ilegamma2 13CH3 sites on a deuterated background.
Devon Sheppard; Chenyun Guo; Vitali Tugarinov
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-10
Journal Detail:
Title:  Journal of biomolecular NMR     Volume:  43     ISSN:  1573-5001     ISO Abbreviation:  J. Biomol. NMR     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2009-03-24     Completed Date:  2009-05-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9110829     Medline TA:  J Biomol NMR     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  229-38     Citation Subset:  IM    
Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA.
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MeSH Terms
Alanine / chemistry*,  metabolism
Carbon Isotopes / chemistry*,  metabolism
Computer Simulation
Escherichia coli / genetics
Isoleucine / chemistry*,  metabolism
Malate Synthase / chemistry
Nuclear Magnetic Resonance, Biomolecular / methods*
Proteins / chemistry*
Pyruvic Acid / metabolism
Valine / chemistry,  metabolism
Reg. No./Substance:
0/Carbon Isotopes; 0/Proteins; 127-17-3/Pyruvic Acid; 56-41-7/Alanine; 7004-03-7/Valine; 73-32-5/Isoleucine; EC Synthase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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