| Methyl-beta-cyclodextrin modified micellar electrokinetic capillary chromatography with laser-induced fluorescence for separation and detection of phospholipids. | |
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MedLine Citation:
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PMID: 11100855 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Micellar electrokinetic capillary chromatography (MECC) with laser-induced fluorescence detection was applied to the separation of amino group-containing phospholipids including phosphatidylethanolamine (PE), phosphatidylserine (PS), lysophosphatidylethanolamine (LysoPE), and lysophosphatidylserine (LysoPS). A fluorogenic dye, 3-(2-furoyl)quinoline-2-carboxaldehyde (FQ), was successfully used to fluorescently label these phospholipids. 4-Fluoro-7-nitrobenzofurazan only produced fluorescent product from LysoPE and PE; signals were not observed from LysoPS and PS. A borax buffer containing sodium deoxycholate modified with methyl-beta-cyclodextrin (methyl-beta-CD) was an excellent MECC system for these phospholipids. Under the optimum conditions, four FQ-labeled phospholipid classes were separated within 8 min. Moreover, each of the PE, PS, LysoPE and LysoPS peaks split into two components corresponding to subclasses with different lengths of the fatty acid chains, but these subclasses were completely resolved only for LysoPE. Detection limits ranged from 0.18 to 1.1 fg (10(-9) - 10(-10) M), which was four- to five-orders of magnitude superior to previously reported CE methods. |
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Authors:
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Le Zhang; S N Krylov; S Hu; N J Dovichi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of chromatography. A Volume: 894 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 2000 Oct |
Date Detail:
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Created Date: 2001-02-20 Completed Date: 2001-03-08 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 129-34 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Alberta, Edmonton, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Micellar Electrokinetic Capillary
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methods* Cyclodextrins / chemistry* Lasers Phospholipids / analysis, isolation & purification* Sensitivity and Specificity Spectrometry, Fluorescence / methods* beta-Cyclodextrins* |
| Chemical | |
Reg. No./Substance:
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0/Cyclodextrins; 0/Phospholipids; 0/beta-Cyclodextrins; 0/methyl-beta-cyclodextrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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