Document Detail


Methotrexate inhibits interleukin-6 production in patients with juvenile rheumatoid arthritis.
MedLine Citation:
PMID:  12739045     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Methotrexate (MTX) is one of the most widely used disease-modifying antirheumatoid drugs in the treatment of juvenile rheumatoid arthritis (JRA). We studied its effect on the production of two proinflammatory cytokines, interleukin-6 (IL-6) and tumor necrosis factor alpha (TNFalpha), by peripheral blood cells in patients with JRA. METHODS: Interleukin-6 and TNFalpha levels were measured at 0 and 4 weeks in whole blood cultures with and without lipopolysaccharide (LPS) stimulation in 19 children treated with MTX (10 mg/m(2 )per week) or placebo. Ten healthy individuals were included as healthy controls. RESULTS: Spontaneous production of IL-6 and TNFalpha by peripheral blood cells of patients with JRA was higher than in healthy controls ( P<0.01). However, IL-6 and TNFalpha production after LPS stimulation was similar in healthy controls and patients. The two groups of patients, i.e., those treated with placebo and those treated with MTX, had similar spontaneous and induced IL-6 and TNFalpha production. At 4 weeks, the drop in spontaneous IL-6 and TNFalpha production was no different in the two groups, but LPS-stimulated IL-6 production was significantly lower in the MTX-treated group than the placebo group ( P<0.05). CONCLUSION: Methotrexate reduces the production of IL-6 by activated cells, and this may be responsible for its anti-inflammatory property.
Authors:
Amita Aggarwal; Ramnath Misra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-03-07
Journal Detail:
Title:  Rheumatology international     Volume:  23     ISSN:  0172-8172     ISO Abbreviation:  Rheumatol. Int.     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-05-09     Completed Date:  2004-01-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8206885     Medline TA:  Rheumatol Int     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  134-7     Citation Subset:  IM    
Affiliation:
Department of Clinical Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, India 226 014. amita@sgpgi.ac.in
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MeSH Terms
Descriptor/Qualifier:
Antirheumatic Agents / therapeutic use*
Arthritis, Juvenile Rheumatoid / drug therapy*,  metabolism
Blood Cells / metabolism
Cells, Cultured
Drug Therapy, Combination
Glucocorticoids / therapeutic use
Humans
Immunosuppressive Agents / therapeutic use*
Interleukin-6 / metabolism*
Lipopolysaccharides / pharmacology
Methotrexate / therapeutic use*
Tumor Necrosis Factor-alpha / metabolism
Chemical
Reg. No./Substance:
0/Antirheumatic Agents; 0/Glucocorticoids; 0/Immunosuppressive Agents; 0/Interleukin-6; 0/Lipopolysaccharides; 0/Tumor Necrosis Factor-alpha; 59-05-2/Methotrexate

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