| Methotrexate-induced senescence in human adenocarcinoma cells is accompanied by induction of p21(waf1/cip1) expression and lack of polyploidy. | |
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MedLine Citation:
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PMID: 19467772 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human colorectal adenocarcinoma C85 cells, treated with high dose methotrexate (1 microM; IC(50)=51 nM), undergo accelerated senescence, as the cells (i) are growth arrested at the G(1) and S phases of the cell cycle, (ii) are SA-beta-galactosidase-positive, (iii) show induced expression of p21(waf1/cip1) and decreased expression of p16(INK4a), and (iv) show DNA synthesis continued at the reduced level. The fraction of C85 cells with DNA content higher than 4N is maintained at the same level (14%) in cells untreated, as well as regrown after the treatment. Multinucleation is found as the main karyotypic abnormality. |
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Authors:
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Magdalena Dabrowska; Grazyna Mosieniak; Janusz Skierski; Ewa Sikora; Wojciech Rode |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-20 |
Journal Detail:
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Title: Cancer letters Volume: 284 ISSN: 1872-7980 ISO Abbreviation: Cancer Lett. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-08-31 Completed Date: 2009-09-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7600053 Medline TA: Cancer Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 95-101 Citation Subset: IM |
Affiliation:
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Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur St., Warsaw 02-093, Poland. m.dabrowska@nencki.gov.pl |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antimetabolites, Antineoplastic
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pharmacology* Cell Aging Cell Cycle / drug effects, physiology* Cell Line, Tumor Cell Survival / drug effects, physiology Cyclin-Dependent Kinase Inhibitor p16 / metabolism Cyclin-Dependent Kinase Inhibitor p21 / metabolism* Humans Methotrexate / pharmacology* Polyploidy* |
| Chemical | |
Reg. No./Substance:
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0/Antimetabolites, Antineoplastic; 0/Cyclin-Dependent Kinase Inhibitor p16; 0/Cyclin-Dependent Kinase Inhibitor p21; 59-05-2/Methotrexate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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