Document Detail


Methotrexate-induced resistance to dactinomycin in choriocarcinoma.
MedLine Citation:
PMID:  3409169     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
NaUCC-2, a choriocarcinoma cell line, was derived from a patient who had a very poor clinical response to combination chemotherapy. Methotrexate (MTX) might have inhibited the antitumor effect of dactinomycin. To investigate this point, in vitro studies were performed to determine the sensitivity and uptake of MTX and dactinomycin (administered individually and in combination) to NaUCC-2 and three other choriocarcinoma cell lines. Dihydrofolate reductase (DHFR) concentrations were studied as well. Although NaUCC-2 showed sensitivity to MTX and dactinomycin, which were comparable to the other cell lines when they were given separately, NaUCC-2 was unique in that the combination of MTX and dactinomycin was less lethal than dactinomycin given by itself. The uptake of MTX in NaUCC-2 was significantly higher than that in the other cell lines, and MTX also induced an increase in dactinomycin uptake in NaUCC-2. There was no significant difference in DHFR activity. Although additional studies are necessary to determine the mechanism responsible for this effect, these findings suggest that a mechanism other than drug uptake or DHFR activity must play a role in the drug resistance for choriocarcinoma. These findings also suggest that the most commonly used combination chemotherapy for choriocarcinoma, dactinomycin and MTX, may not always be the best method.
Authors:
S Goto; Y Okayama; C Fan; S Ueda; M Saito; Y Furuhashi; T Ishizuka; Y Tomoda
Related Documents :
3760769 - Kinetics of oxygen uptake and release by red blood cells of chicken and duck.
1594669 - Iron, transferrin, and haptoglobin levels after a single bout of exercise in men.
1373979 - Effects of periodic backflushing on ultrafiltration performance.
6494799 - Ear drum mobility and middle ear volume measured with tympanometry.
7308589 - The lipocytes in normal human liver. a quantitative study.
11928919 - Exsanguination of lower limbs in healthy male subjects.
9309629 - Pre-exercise branched-chain amino acid administration increases endurance performance i...
17081809 - Gaseous metabolism of the chicken embryo and hatchling during post-hypoxic recovery.
7605249 - Electromyographic signal changes during exercise in human chronic jaw-muscle pain.
Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer     Volume:  62     ISSN:  0008-543X     ISO Abbreviation:  Cancer     Publication Date:  1988 Sep 
Date Detail:
Created Date:  1988-09-28     Completed Date:  1988-09-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0374236     Medline TA:  Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  873-7     Citation Subset:  AIM; IM    
Affiliation:
Department of Obstetrics and Gynecology, School of Medicine, Nagoya University, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Aneuploidy
Antineoplastic Combined Chemotherapy Protocols
Biological Transport
Choriocarcinoma / drug therapy*,  genetics
Dactinomycin* / administration & dosage,  metabolism
Drug Resistance*
Female
Humans
Methotrexate / administration & dosage*,  metabolism
Tetrahydrofolate Dehydrogenase / metabolism
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
50-76-0/Dactinomycin; 59-05-2/Methotrexate; EC 1.5.1.3/Tetrahydrofolate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Plasma tetranectin is reduced in cancer and related to metastasia.
Next Document:  Target organ disposition and plasma pharmacokinetics of doxorubicin incorporated into albumin micros...