| Methotrexate-loaded chitosan- and glycol chitosan-based nanoparticles: a promising strategy for the administration of the anticancer drug to brain tumors. | |
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MedLine Citation:
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PMID: 21948322 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Brain tumor treatment employing methotrexate (MTX) is limited by the efflux mechanism of Pg-p on the blood-brain barrier. We aimed to investigate MTX-loaded chitosan or glycol chitosan (GCS) nanoparticles (NPs) in the presence and in the absence of a coating layer of Tween 80 for brain delivery of MTX. The effect of a low Tween 80 concentration was evaluated. MTX NPs were formulated following the ionic gelation technique and size and zeta potential measurements were acquired. Transport across MDCKII-MDR1 monolayer and cytotoxicity studies against C6 glioma cell line were also performed. Cell/particles interaction was visualized by confocal microscopy. The particles were shown to be cytotoxic against C6 cells line and able to overcome MDCKII-MDR1 cell barrier. GCS-based NPs were the most cytotoxic NPs. Confocal observations highlighted the internalization of Tween 80-coated fluorescent NPs more than Tween 80-uncoated NPs. The results suggest that even a low concentration of Tween 80 is sufficient for enhancing the transport of MTX from the NPs across MDCKII-MDR1 cells. The nanocarriers represent a promising strategy for the administration of MTX to brain tumors which merits further investigations under in vivo conditions. |
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Authors:
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Adriana Trapani; Nunzio Denora; Giuliano Iacobellis; Johannes Sitterberg; Udo Bakowsky; Thomas Kissel |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-09-27 |
Journal Detail:
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Title: AAPS PharmSciTech Volume: 12 ISSN: 1530-9932 ISO Abbreviation: AAPS PharmSciTech Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-11-29 Completed Date: 2012-03-26 Revised Date: 2012-10-01 |
Medline Journal Info:
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Nlm Unique ID: 100960111 Medline TA: AAPS PharmSciTech Country: United States |
Other Details:
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Languages: eng Pagination: 1302-11 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical Chemistry, School of Pharmacy, University of Bari, Italy. atrapani@farmchim.uniba.it |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antimetabolites, Antineoplastic / administration & dosage, chemistry, metabolism* Blood-Brain Barrier / metabolism Brain Neoplasms / metabolism*, pathology Capillary Permeability Cell Line Cell Survival / drug effects Chemistry, Pharmaceutical Chitosan / analogs & derivatives, chemistry* Dogs Dose-Response Relationship, Drug Drug Carriers* Drug Compounding Glycols / chemistry* Kinetics Methotrexate / administration & dosage, chemistry, metabolism* Microscopy, Atomic Force Microscopy, Confocal Nanoparticles* Nanotechnology* P-Glycoprotein / genetics, metabolism Particle Size Polysorbates / chemistry Rats Solubility Technology, Pharmaceutical / methods* Transfection |
| Chemical | |
Reg. No./Substance:
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0/Antimetabolites, Antineoplastic; 0/Drug Carriers; 0/Glycols; 0/P-Glycoprotein; 0/Polysorbates; 59-05-2/Methotrexate; 9012-76-4/Chitosan |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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